Título : Role of aryl hydrocarbon receptor (AHR) in overall retinoid metabolism: Response comparisons to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure between wild-type and AHR knockout mice |
Autor : Esteban Mozo, Javier Sánchez Pérez, Ismael Hamscher, Gerd  Miettinen, Hanna  Korkalainen, Merja  Viluksela, Matti  Pohjanvirta, Raimo  Hakansson, Helen |
Editor : Elsevier |
Departamento: Departamentos de la UMH::Biología Aplicada |
Fecha de publicación: 2021-02-16 |
URI : https://hdl.handle.net/11000/37573 |
Resumen :
Young adult wild-type and aryl hydrocarbon receptor knockout (AHRKO) mice of both sexes and the C57BL/6J
background were exposed to 10 weekly oral doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; total dose of
200 μg/kg bw) to further characterize the observed impacts of AHR as well as TCDD on the retinoid system.
Unexposed AHRKO mice harboured heavier kidneys, lighter livers and lower serum all-trans retinoic acid (ATRA)
and retinol (REOH) concentrations than wild-type mice. Results from the present study also point to a role for the
murine AHR in the control of circulating REOH and ATRA concentrations. In wild-type mice, TCDD elevated liver
weight and reduced thymus weight, and drastically reduced the hepatic concentrations of 9-cis-4-oxo-13,14-
dihydro-retinoic acid (CORA) and retinyl palmitate (REPA). In female wild-type mice, TCDD increased the hepatic concentration of ATRA as well as the renal and circulating REOH concentrations. Renal CORA concentrations were substantially diminished in wild-type male mice exclusively following TCDD-exposure, with a
similar tendency in serum. In contrast, TCDD did not affect any of these toxicity or retinoid system parameters in
AHRKO mice. Finally, a distinct sex difference occurred in kidney concentrations of all the analysed retinoid
forms. Together, these results strengthen the evidence of a mandatory role of AHR in TCDD-induced retinoid
disruption, and suggest that the previously reported accumulation of several retinoid forms in the liver of AHRKO
mice is a line-specific phenomenon. Our data further support participation of AHR in the control of liver and
kidney development in mice.
|
Palabras clave/Materias: Aryl hydrocarbon receptor 2,3,7,8-Tetrachlorodibenzo-p-dioxin TCDD Genetically modified organisms Retinoids Vitamin A |
Área de conocimiento : CDU: Ciencias puras y naturales: Biología |
Tipo de documento : info:eu-repo/semantics/article |
Derechos de acceso: info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI : https://doi.org/10.1016/j.reprotox.2021.02.004 |
Publicado en: Reproductive Toxicology, Volume 101, April 2021, Pages 33-49 |
Aparece en las colecciones: Artículos Biología Aplicada
|