Título : Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits |
Autor : Hadjadj, Imane Fabova, Zuzana Garcia Pardo, Maria Luz Agea, Iván  Loncová, Barbora  Morovic, Martin  Makovicky, Peter  Argente, María José  Sirotkin, Alexander V. |
Editor : MDPI |
Departamento: Departamentos de la UMH::Tecnología Agroalimentaria |
Fecha de publicación: 2025-02 |
URI : https://hdl.handle.net/11000/36551 |
Resumen :
The aim of this study is to examine the influence of food restriction on rabbit
ovarian functions. A total of eight females were fed ad libitum (NF), while eight females
were subjected to 50% food restriction (RF). One month later, all females were euthanized.
Weights and lengths of ovaries and uterine horns were measured. Representative parts of
the ovaries were subjected to histomorphometry analysis of folliculogenesis. Granulosa
cells were isolated and cell viability, proliferation (accumulation of PCNA, cyclin B1, and
BrdU-positive cells), apoptosis (accumulation of bax, caspase 3, and DNA fragmentation)
were evaluated. Granulosa cells were subjected to proteomic analysis by using the nano
HPLC-Chip-MS/MS method. Estradiol and progesterone release by ovarian and granulosa
cells was assessed by ELISA. Ovarian and uterine horn weights were lower in RF than
NF. The diameter of follicles and oocytes and the thickness of the theca and granulosa
cells were higher in RF than NF. RF showed a lower percentage of cells containing bax
and caspase 3, occurrence of DNA fragmented cells, and estradiol and progesterone. RF
had higher incorporation of BrdU, a higher proportion of cells containing PCNA and
cyclin B1, and a lower percentage of viable cells. RF produced more specific proteins than
NF, including peptides involved in cell differentiation, proliferation/division, mitotic cell
cycle, and GTP-ase activity. In conclusion, food restriction can activate reproduction by
(1) selection of the growing primordial follicles, (2) better transformation of secondary to
preovulatory follicles, (3) increasing growth of oocytes, (4) increasing proliferation and decreasing apoptosis in granulosa cells, (5) changes in ovarian secretory activity, and (6)
changes in the number of peptides.
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Palabras clave/Materias: food restriction apoptosis hormone ovarian follicle rabbit |
Tipo de documento : info:eu-repo/semantics/article |
Derechos de acceso: info:eu-repo/semantics/openAccess |
DOI : https://doi.org/10.3390/ani15091282 |
Aparece en las colecciones: Artículos Tecnología Agroalimentaria
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