Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/30708

Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression


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Title:
Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression
Authors:
Hernández Ortego, Pablo
Torres Montero, María Remedios
De la Peña García, Elvira  
Viana de la Iglesia, Félix
Fernández Trillo, Jorge
Editor:
MDPI [Commercial Publisher]
Department:
Instituto de Neurociencias
Departamentos de la UMH::Fisiología
Issue Date:
2022-12-18
URI:
https://hdl.handle.net/11000/30708
Abstract:
TRPM8 is a non-selective cation channel expressed in primary sensory neurons and other tissues, including the prostate and urothelium. Its participation in different physiological and pathological processes such as thermoregulation, pain, itch, inflammation and cancer has been widely described, making it a promising target for therapeutic approaches. The detection and quantification of TRPM8 seems crucial for advancing the knowledge of the mechanisms underlying its role in these pathophysiological conditions. Antibody-based techniques are commonly used for protein detection and quantification, although their performance with many ion channels, including TRPM8, is suboptimal. Thus, the search for reliable antibodies is of utmost importance. In this study, we characterized the performance of six TRPM8 commercial antibodies in three immunodetection techniques: Western blot, immunocytochemistry and immunohistochemistry. Different outcomes were obtained for the tested antibodies; two of them proved to be successful in detecting TRPM8 in the three approaches while, in the conditions tested, the other four were acceptable only for specific techniques. Considering our results, we offer some insight into the usefulness of these antibodies for the detection of TRPM8 depending on the methodology of choice.
Keywords/Subjects:
TRPM8
Western blot
immunocytochemistry
immunohistochemistry
Type of document:
application/pdf
Access rights:
info:eu-repo/semantics/openAccess
DOI:
https://doi.org/10.3390/ijms232416164
Appears in Collections:
Artículos Fisiología



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