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https://hdl.handle.net/11000/30708
Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression
Title: Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression |
Authors: Hernández Ortego, Pablo Torres Montero, María Remedios De la Peña García, Elvira ![](/jspui/image/ORCID.jpg) Viana de la Iglesia, Félix Fernández Trillo, Jorge |
Editor: MDPI [Commercial Publisher] |
Department: Instituto de Neurociencias Departamentos de la UMH::Fisiología |
Issue Date: 2022-12-18 |
URI: https://hdl.handle.net/11000/30708 |
Abstract:
TRPM8 is a non-selective cation channel expressed in primary sensory neurons and other
tissues, including the prostate and urothelium. Its participation in different physiological and
pathological processes such as thermoregulation, pain, itch, inflammation and cancer has been
widely described, making it a promising target for therapeutic approaches. The detection and
quantification of TRPM8 seems crucial for advancing the knowledge of the mechanisms underlying
its role in these pathophysiological conditions. Antibody-based techniques are commonly used for
protein detection and quantification, although their performance with many ion channels, including
TRPM8, is suboptimal. Thus, the search for reliable antibodies is of utmost importance. In this study,
we characterized the performance of six TRPM8 commercial antibodies in three immunodetection
techniques: Western blot, immunocytochemistry and immunohistochemistry. Different outcomes
were obtained for the tested antibodies; two of them proved to be successful in detecting TRPM8 in
the three approaches while, in the conditions tested, the other four were acceptable only for specific
techniques. Considering our results, we offer some insight into the usefulness of these antibodies for
the detection of TRPM8 depending on the methodology of choice.
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Keywords/Subjects: TRPM8 Western blot immunocytochemistry immunohistochemistry |
Type of document: application/pdf |
Access rights: info:eu-repo/semantics/openAccess |
DOI: https://doi.org/10.3390/ijms232416164 |
Appears in Collections: Artículos Fisiología
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