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dc.contributor.authorHernández Ortego, Pablo-
dc.contributor.authorTorres Montero, María Remedios-
dc.contributor.authorDe la Peña García, Elvira-
dc.contributor.authorViana de la Iglesia, Félix-
dc.contributor.authorFernández Trillo, Jorge-
dc.contributor.otherInstituto de Neurocienciases_ES
dc.contributor.otherDepartamentos de la UMH::Fisiologíaes_ES
dc.date.accessioned2024-01-26T10:22:27Z-
dc.date.available2024-01-26T10:22:27Z-
dc.date.created2022-12-18-
dc.identifier.citationInternational journal of molecular sciences 2022 Dec 18;23(24):16164es_ES
dc.identifier.issn1422-0067 (Electronic)-
dc.identifier.urihttps://hdl.handle.net/11000/30708-
dc.description.abstractTRPM8 is a non-selective cation channel expressed in primary sensory neurons and other tissues, including the prostate and urothelium. Its participation in different physiological and pathological processes such as thermoregulation, pain, itch, inflammation and cancer has been widely described, making it a promising target for therapeutic approaches. The detection and quantification of TRPM8 seems crucial for advancing the knowledge of the mechanisms underlying its role in these pathophysiological conditions. Antibody-based techniques are commonly used for protein detection and quantification, although their performance with many ion channels, including TRPM8, is suboptimal. Thus, the search for reliable antibodies is of utmost importance. In this study, we characterized the performance of six TRPM8 commercial antibodies in three immunodetection techniques: Western blot, immunocytochemistry and immunohistochemistry. Different outcomes were obtained for the tested antibodies; two of them proved to be successful in detecting TRPM8 in the three approaches while, in the conditions tested, the other four were acceptable only for specific techniques. Considering our results, we offer some insight into the usefulness of these antibodies for the detection of TRPM8 depending on the methodology of choice.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent19es_ES
dc.language.isoenges_ES
dc.publisherMDPI [Commercial Publisher]es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTRPM8es_ES
dc.subjectWestern blotes_ES
dc.subjectimmunocytochemistryes_ES
dc.subjectimmunohistochemistryes_ES
dc.titleValidation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expressiones_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.contributor.instituteInstitutos de la UMH::Instituto de Neurocienciases_ES
dc.relation.publisherversionhttps://doi.org/10.3390/ijms232416164es_ES
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