Biochemical Profiling and Bioactivity of Five Selected MicroalgaeSpecies as Potential Sources of Bioactive Compounds forNutritional and Biotechnological Applications

Abstract

Microalgae have emerged as promising sustainable sources of bioactive compounds, essential for addressing global challenges. Tis study investigated the biochemical profles of fve microalgae species: Nannochloropsis sp., Tetraselmis chuii, Chaetoceros muelleri, Talassiosira weissfogii, and Tisochrysis lutea. By using NMR spectroscopy, organic acids, amino acids, and other compounds were revealed. T. chuii was particularly rich in acetate, whereas the main organic acid in T. lutea was lactate. ICP-MS analysis indicated substantial variations in elemental concentrations across the species, with T. chuii showing the highest calcium content and C. muelleri having the highest iron content. SPME-GC/MS revealed that alcohols, acids, and aldehydes were the principal volatiles. Fatty acid profle was described by GC-MS technique. Carotenoid profling highlighted T. lutea as having the highest total carotenoid content. Antioxidant activities were evaluated with C. muelleri demonstrating superior efcacy. Furthermore, the microalgae demonstrated moderate to signifcant activity against both bacterial and fungal pathogens. In addition, T. lutea and Nannochloropsis sp. demonstrate signifcant antibioflm activity against various bacterial strains. Tis study highlights the potential of these microalgae as valuable sources of diverse metabolites contributing to nutritional and biotechnological advancements and addressing global food and health challenges