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Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5

Título :
Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
Autor :
Nieves-Cordones, Manuel  
Lara, Alberto
Silva, Martha
Amo Pérez, Jesús  
Rodriguez-Sepulveda, Pascual
Rivero, Rosa M.
martinez, vicente  
Botella, M. Ángeles  
Rubio, Francisco
Editor :
Wiley
Departamento:
Departamentos de la UMH::Biología Aplicada
Fecha de publicación:
2020-03
URI :
https://hdl.handle.net/11000/32435
Resumen :
Root K+ acquisition is a key process for plant growth and development, extensively studied in the model plant Arabidopsis thaliana. Because important differences may exist among species, translational research supported by specific studies is needed in crops such as tomato. Here we present a reverse genetics study to demonstrate the role of the SlHAK5 K+ transporter in tomato K+ nutrition, Cs+ accumulation and its fertility. slhak5 KO lines, generated by CRISPR-Cas edition, were characterized in growth experiments, Rb+ and Cs+ uptake tests and root cells K+-induced plasma membrane depolarizations. Pollen viability and its K+ accumulation capacity were estimated by using the K+-sensitive dye Ion Potassium Green 4. SlHAK5 is the major system for high-affinity root K+ uptake required for plant growth at low K+, even in the presence of salinity. It also constitutes a pathway for Cs+ entry in tomato plants with a strong impact on fruit Cs+ accumulation. SlHAK5 also contributes to pollen K+ uptake and viability and its absence produces almost seedless fruits. Knowledge gained into SlHAK5 can serve as a model for other crops with fleshy fruits and it can help to generate tools to develop low Cs+ or seedless fruits crops.
Palabras clave/Materias:
caesium
pollen
potassium
salinity
transport
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI :
https://doi.org/10.1111/pce.13769
Aparece en las colecciones:
Artículos Biología Aplicada



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.