Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/30739
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dc.contributor.authorDe la Peña García, Elvira-
dc.contributor.authorGomis García, Ana María-
dc.contributor.otherDepartamentos de la UMH::Fisiologíaes_ES
dc.contributor.otherInstituto de Neurocienciases_ES
dc.date.accessioned2024-01-26T10:48:26Z-
dc.date.available2024-01-26T10:48:26Z-
dc.date.created2019-
dc.identifier.citationTRP Channels Methods and Protocols Edited by Antonio Ferrer-Montiel Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, Alicante, Spain Tim Hucho Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9445-8 ISBN 978-1-4939-9446-5 (eBook) © Springer Science+Business Media, LLC, part of Springer Nature 2019es_ES
dc.identifier.isbn978-1-4939-9445-8-
dc.identifier.isbn978-1-4939-9446-5 (eBook)-
dc.identifier.issn1940-6029 (electronic)-
dc.identifier.issn1064-3745-
dc.identifier.urihttps://hdl.handle.net/11000/30739-
dc.description.abstractTransient receptor potential (TRP) ion channels are involved in a variety of fundamental physiological processes, and their malfunction produces numerous human diseases. Therefore, these proteins represent a class of attractive drug targets and a class of important off-targets for in vitro pharmacological profiling. In the past decades, the rapid progress in emerging functional assays and instrumentation has enabled to readily monitor thermoTRP channel activity, and to develop high throughput screening (HTS) assays for TPR drug discovery. Chronologically, functional methods for ion channels include the ligand binding assay, flux-based assay, electrophysiology, fluorescence-based assays, and, more recently, automated electrophysiological assays. Here we described the methodology used to monitor the functionality of two thermoTRPs, TRPV1 and TRPM8, based on Ca2+ microfluorography using a 96-well fluorescence plate reader that allows the implementation of a medium- to high-throughput format ideal for drug screening.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent15es_ES
dc.language.isoenges_ES
dc.publisherHumana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature.es_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectIon channelses_ES
dc.subjectHigh-throughput screeninges_ES
dc.subjectLigand binding assayes_ES
dc.subjectFlux-based assayes_ES
dc.subjectFluorescence- based assayes_ES
dc.subjectDrug discoveryes_ES
dc.titleChapter 6: Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Techniquees_ES
dc.typeinfo:eu-repo/semantics/bookPartes_ES
dc.relation.publisherversionhttps://doi.org/10.1007/978-1-4939-9446-5es_ES
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