Prediction of sequence-dependent and mutational effects on the aggregation of peptides and proteins

Abstract

We have developed a statistical mechanics algorithm, TANGO, to predict protein aggregation. TANGO is based on the physico-chemical principles of b-sheet formation, extended by the assumption that the core regions of an aggregate are fully buried. Our algorithm accurately predicts the aggregation of a data set of 179 peptides compiled from the literature as well as of a new set of 71 peptides derived from human diseaserelated proteins, including prion protein, lysozyme and b2-microglobulin. TANGO also correctly predicts pathogenic as well as protective mutations of the Alzheimer b-peptide, human lysozyme and transthyretin, and discriminates between b-sheet propensity and aggregation. Our results confirm the model of intermolecular b-sheet formation as a widespread underlying mechanism of protein aggregation. Furthermore, the algorithm opens the door to a fully automated, sequence-based design strategy to improve the aggregation properties of proteins of scientific or industrial interest.