Título : Influence of Heme Oxygenase 1 Modulation on the Progression
of Murine Collagen-Induced Arthritis |
Autor : Devesa Giner, Isabel  Ferrándiz, Maria Luisa Terencio, M. Carmen  Joosten, Leo  van den Berg, Wim  Alcaraz, María José |
Editor : American College of Rheumatology |
Departamento: Departamentos de la UMH::Bioquímica y Biología Molecular |
Fecha de publicación: 2005 |
URI : https://hdl.handle.net/11000/36051 |
Resumen :
Objective. Heme oxygenase 1 (HO-1) can be induced
by inflammatory mediators as an adaptive response.
The objective of the present study was to
determine the consequences of HO-1 modulation in the
murine collagen-induced arthritis (CIA) model.
Methods. DBA/1J mice were treated with an inhibitor
of HO-1, tin protoporphyrin IX (SnPP), or with
an inducer of HO-1, cobalt protoporphyrin IX (CoPP),
from day 22 to day 29 after CIA induction. The clinical
evolution of disease was monitored visually. At the end
of the experiment, joints were examined for histopathologic
changes. Cytokine levels in paws were measured by
enzyme-linked immunosorbent assay. Levels of HO-1,
cyclooxygenase 2 (COX-2), and prostaglandin E2
(PGE2) were determined. Effects of treatments on the
early phase of disease and after prophylactic administration
were also assessed.
Results. CoPP strongly induced HO-1, resulting in
the inhibition of cartilage erosion accompanied by extensive
fibrosis in the joint. Levels of tumor necrosis factor
(TNF ), interleukin-2 (IL-2), and IL-10 were inhibited by
CoPP, whereas levels of vascular endothelial growth factor
were increased. Treatment with SnPP significantly reduced
the severity of CIA, with inhibition of joint inflammation
and cartilage destruction. The levels of PGE2,
IL-1 , and TNF were also significantly reduced by SnPP
treatment, which did not modify COX-2 protein expression.
SnPP was more effective than CoPP in preventing the
development of CIA (prophylactic administration)
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Área de conocimiento : CDU: Ciencias puras y naturales: Biología: Bioquímica. Biología molecular. Biofísica |
Tipo de documento : info:eu-repo/semantics/article |
Derechos de acceso: info:eu-repo/semantics/closedAccess |
DOI : https://doi.org/10.1002/art.21356 |
Aparece en las colecciones: Artículos Bioquímica y Biología Molecular
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