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Influence of Heme Oxygenase 1 Modulation on the Progression of Murine Collagen-Induced Arthritis


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Título :
Influence of Heme Oxygenase 1 Modulation on the Progression of Murine Collagen-Induced Arthritis
Autor :
Devesa Giner, Isabel  
Ferrándiz, Maria Luisa
Terencio, M. Carmen  
Joosten, Leo  
van den Berg, Wim  
Alcaraz, María José
Editor :
American College of Rheumatology
Departamento:
Departamentos de la UMH::Bioquímica y Biología Molecular
Fecha de publicación:
2005
URI :
https://hdl.handle.net/11000/36051
Resumen :
Objective. Heme oxygenase 1 (HO-1) can be induced by inflammatory mediators as an adaptive response. The objective of the present study was to determine the consequences of HO-1 modulation in the murine collagen-induced arthritis (CIA) model. Methods. DBA/1J mice were treated with an inhibitor of HO-1, tin protoporphyrin IX (SnPP), or with an inducer of HO-1, cobalt protoporphyrin IX (CoPP), from day 22 to day 29 after CIA induction. The clinical evolution of disease was monitored visually. At the end of the experiment, joints were examined for histopathologic changes. Cytokine levels in paws were measured by enzyme-linked immunosorbent assay. Levels of HO-1, cyclooxygenase 2 (COX-2), and prostaglandin E2 (PGE2) were determined. Effects of treatments on the early phase of disease and after prophylactic administration were also assessed. Results. CoPP strongly induced HO-1, resulting in the inhibition of cartilage erosion accompanied by extensive fibrosis in the joint. Levels of tumor necrosis factor (TNF ), interleukin-2 (IL-2), and IL-10 were inhibited by CoPP, whereas levels of vascular endothelial growth factor were increased. Treatment with SnPP significantly reduced the severity of CIA, with inhibition of joint inflammation and cartilage destruction. The levels of PGE2, IL-1 , and TNF were also significantly reduced by SnPP treatment, which did not modify COX-2 protein expression. SnPP was more effective than CoPP in preventing the development of CIA (prophylactic administration)
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología: Bioquímica. Biología molecular. Biofísica
Tipo de documento :
info:eu-repo/semantics/article
Derechos de acceso:
info:eu-repo/semantics/closedAccess
DOI :
https://doi.org/10.1002/art.21356
Aparece en las colecciones:
Artículos Bioquímica y Biología Molecular



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