Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/35460

Sputum Microbiome Dynamics in Chronic Obstructive Pulmonary Disease Patients during an Exacerbation Event and Post-Stabilization


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Title:
Sputum Microbiome Dynamics in Chronic Obstructive Pulmonary Disease Patients during an Exacerbation Event and Post-Stabilization
Authors:
LOPEZ CARO, JUAN CARLOS  
Santibáñez, Miguel  
GARCIA RIVERO, JUAN LUIS  
Villanueva, Manuel
Sáinz, Jesús
González Astorqui, Pablo
Hierro, Margarita
Rodríguez Porres, Mariano
Parás Bravo, Paula  
Mira, Alex
RODRIGUEZ DIAZ, JUAN CARLOS  
Galiana, Antonio  
Editor:
Karger Publishers
Department:
Departamentos de la UMH::Producción Vegetal y Microbiología
Issue Date:
2019-08-22
URI:
https://hdl.handle.net/11000/35460
Abstract:
Background: Chronic obstructive pulmonary disease (COPD) affects up to 65 million people worldwide, and COPD exacerbation causes tissue damage and subsequent loss of lung function. It is a multifactorial event in which respiratory infections are involved, but little is known about its dynamics. Objectives: The objective of our study was to determine the microbiome composition during an exacerbation event and post-stabilization. Methods: We conducted an observational analytical study of a cohort of 55 COPD patients in which 2 sputum samples (the first taken during an exacerbation event and the second during clinical post-stabilization) were submitted to 16s RNA ribosomal analysis by Illumina Miseq Next Generation Sequencing (NGS). The presence of respiratory viruses was also determined. Results: Our study found a stable microbiome composition in the post-stabilization sputum samples of COPD patients, and 4 additional microbiomes in samples taken during the exacerbation, 3 of which showed a marked dysbiosis by Haemophilus, Pseudomonas, and Serratia. The fourth exacerbation microbiome had a very similar composition to post-stabilization samples, but some pathogens such as Moraxella and respiratory viruses were also found. Conclusions: Our study reveals the main protagonists involved in lung microbiome dynamics during an exacerbation event and post-stabilization in COPD patients by NGS analysis
Keywords/Subjects:
Chronic obstructive pulmonary disease
Exacerbation
Microbioma
Type of document:
info:eu-repo/semantics/article
Access rights:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI:
https://doi.org/10.1159/000501988
Appears in Collections:
Artículos Producción vegetal y microbiología



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