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Polyfluorene-Based Multicolor Fluorescent Nanoparticles Activated by Temperature for Bioimaging and Drug Delivery


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Title:
Polyfluorene-Based Multicolor Fluorescent Nanoparticles Activated by Temperature for Bioimaging and Drug Delivery
Authors:
Rubio-Camacho, Marta  
Alacid Martínez, Yolanda Inmaculada  
Mallavia, Ricardo  
Martínez Tomé, María José
Mateo , C. Reyes  
Editor:
MDPI
Department:
Departamentos de la UMH::Agroquímica y Medio Ambiente
Issue Date:
2019-10-15
URI:
https://hdl.handle.net/11000/30729
Abstract:
Multifunctional nanoparticles have been attracting growing attention in recent years because of their capability to integrate materials with di erent features in one entity, which leads them to be considered as the next generation of nanomedicine. In this work, we have taken advantage of the interesting properties of conjugated polyelectrolytes to develop multicolor fluorescent nanoparticles with integrating imaging and therapeutic functionalities. With this end, thermosensitive liposomes were coated with three recently synthesized polyfluorenes: copoly-((9,9-bis(60-N,N,N-trimethylammonium)hexyl)-2,7-(fluorene)-alt-1,4-(phenylene)) bromide (HTMA-PFP), copoly-((9,9-bis(60-N,N,N-trimethylammonium)hexyl)-2,7-(fluorene)-alt-4,7-(2- (phenyl)benzo(d) (1,2,3) triazole)) bromide (HTMA-PFBT) and copoly-((9,9-bis(60-N,N,Ntrimethylammonium) hexyl)-2,7-(fluorene)-alt-1,4-(naphtho(2,3c)-1,2,5-thiadiazole)) bromide (HTMA-PFNT), in order to obtain blue, green and red fluorescent drug carriers, respectively. The stability, size and morphology of the nanoparticles, as well as their thermotropic behavior and photophysical properties, have been characterized by Dynamic Light Scattering (DLS), Zeta Potential, transmission electron microscope (TEM) analysis and fluorescence spectroscopy. In addition, the suitability of the nanostructures to carry and release their contents when triggered by hyperthermia has been explored by using carboxyfluorescein as a hydrophilic drug model. Finally, preliminary experiments with mammalian cells demonstrate the capability of the nanoparticles to mark and visualize cells with di erent colors, evidencing their potential use for imaging and therapeutic applications.
Keywords/Subjects:
multifunctional fluorescent nanoparticles
conjugated polyelectrolytes (CPEs)
thermosensitive liposomes (TSLs)
bioimaging
drug carrier
release experiments
Knowledge area:
CDU: Ciencias puras y naturales: Química
Type of document:
application/pdf
Access rights:
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI:
https://doi.org/10.3390/nano9101485
Appears in Collections:
Artículos Agroquímica y Medio Ambiente



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