Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/6993
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dc.contributor.authorNombela Díaz, Iván-
dc.contributor.authorCarrión Pérez, Lourdes Aurora-
dc.contributor.authorPuente Marín, Sara-
dc.contributor.authorChico, Verónica-
dc.contributor.authorMercado Cerda, Luis Antonio-
dc.contributor.authorPérez, Luis-
dc.contributor.authorColl Morales, Julio-
dc.contributor.authorOrtega-Villaizan Romo, María del Mar-
dc.date.accessioned2021-01-25T09:55:16Z-
dc.date.available2021-01-25T09:55:16Z-
dc.date.created2017-12-13-
dc.date.issued2021-01-25-
dc.identifier.issn2046-1402-
dc.identifier.urihttp://hdl.handle.net/11000/6993-
dc.description.abstractBackground: Some fish viruses, such as piscine orthoreovirus and infectious salmon anemia virus, target red blood cells (RBCs), replicate inside them and induce an immune response. However, the roles of RBCs in the context of infectious pancreatic necrosis virus (IPNV) infection have not been studied yet. Methods: Ex vivo rainbow trout RBCs were obtained from peripheral blood, Ficoll purified and exposed to IPNV in order to analyze infectivity and immune response using RT-qPCR, immune fluorescence imaging, flow cytometry and western-blotting techniques. Results: IPNV could not infect RBCs; however, IPNV increased the expression of the INF1-related genes ifn-1, pkr and mx genes. Moreover, conditioned media from IPNV-exposed RBCs conferred protection against IPNV infection in CHSE-214 fish cell line. Conclusions: Despite not being infected, rainbow trout RBCs could respond to IPNV with increased expression of antiviral genes. Fish RBCs could be considered as mediators of the antiviral response and therefore targets of new strategies against fish viral infections. Further research is ongoing to completely understand the molecular mechanism that triggers this antiviral response in rainbow trout RBCs.es
dc.description.sponsorshipThis work was supported by the European Research Council (ERC starting grant 2014 GA639249)-
dc.formatapplication/pdfes
dc.format.extent26es
dc.language.isoenges
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjecterythrocyteses
dc.subjectIPNVes
dc.subjectbirnaviruses
dc.subjectimmune responsees
dc.subjectantivirales
dc.subjecttroutes
dc.subjectinterferones
dc.subject.other577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleInfectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective [version 2; referees: 2 approved]es
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.12688/f1000research.12994.2-
dc.relation.publisherversionhttps://doi.org/10.12688/f1000research.12994.2-
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Instituto de Biología Molecular y Celular


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