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Fenómenos de interferencia viral en células de cipriniformes y perciformes, dos grupos de peces de importancia en acuicultura


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Título :
Fenómenos de interferencia viral en células de cipriniformes y perciformes, dos grupos de peces de importancia en acuicultura
Autor :
Codina Márquez, Helena
Tutor:
Pérez García-Estañ, Luis
Fecha de publicación:
2017-07-12
URI :
http://hdl.handle.net/11000/4166
Resumen :
Estudios previos con la línea celular EPC de carpa (cipriniformes), persistentemente infectada con IPNV (EPCIPNV), mostraron que dicha línea es resistente a la infección por virus heterólogos, como VHSV, y que secreta al medio factores con actividad antiviral. En el presente trabajo se perseguía ob...  Ver más
Previous studies with the cell line EPC from carp (cyprinid), persistently infected with IPNV (EPCIPNV), revealed that this line is resistant to heterologous viruses infection, such as VHSV, and that these cells secrete factors with antiviral activity. In this project, the objective was to obtain another cell line persistently infected with IPNV from a different group of fish. To do that, a cell line from gilthead sea bream (perciform) was used (SAFIPNV). It was found that, although this line is less stable, its behavior related to VHSV superinfection resistance is similar to those from EPCIPNV. Moreover, here we show experimental evidence of the presence of antiviral factors in the supernatant of EPCIPNV y SAFIPNV cultures that induce resistance to VHSV infection. Quantitative PCR analysis pointed that mx gene, induced by Interferon, could play a role in the establishment of the antiviral state, possibly through the Interferon type I system. This studies help understand the innate immune response mechanisms, which would contribute to the development and improvement of vaccines against fish viruses’.
Palabras clave/Materias:
virus de pez
persistencia
interferencia viral
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología: Bioquímica. Biología molecular. Biofísica
Tipo de documento :
info:eu-repo/semantics/bachelorThesis
Derechos de acceso:
info:eu-repo/semantics/openAccess
Aparece en las colecciones:
TFG - Biotecnología



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