Title: Polyphenolic extracts from Diospyros kaki and Vitis vinifera by-products stimulate cytoprotective effects in bacteria-cell host interactions by mediation of transcription factor Nrf2 |
Authors: Martínez-Madrid, Mª Concepción Moreno Chamba, Bryan Mauricio Salazar, Julio  Narváez Asensio, Marta Navarro Simarro, Pablo  Saura, Domingo  Neacsu, Madalina Martí, Nuria  Valero, Manuel |
Editor: Elsevier |
Department: Departamentos de la UMH::Agroquímica y Medio Ambiente |
Issue Date: 2024-09 |
URI: https://hdl.handle.net/11000/35893 |
Abstract:
Background: The intestinal and skin epithelium play a strong role against bacterial stimuli which leads to
inflammation and oxidative stress when overwhelmed. Polyphenols from fruit-rich diets and by-products show
promise against bacterial deleterious effects; however, their antibacterial and health-promoting effects remain
understudied.
Purpose: This study aimed to assess the impact of polyphenolic extracts of grape (GrPE), persimmon (PePE) and
pomegranate (PoPE) by-products on bacterial pathogen-host interactions, focusing beyond growth inhibition to
explore their effects on bacterial adhesion, invasion, and modulation of host responses.
Methods: The microdilution method, as well as the tetrazolium based MTT cell proliferation and cytotoxicity
assay with crystal violet staining were used to identify extracts sub-inhibitory concentrations that interfere with
bacterial adhesion, invasion or lipopolysaccharides (LPS) effect on cell hosts without compromising host
viability. The cytoprotective effects of extracts were assessed in a knock-down model of nuclear factor erythroid
2-related factor 2 (Nrf2).
Results: All extracts demonstrated significant reductions in pathogen adhesion to Caco-2 and HaCaT cells while
preserving cellular integrity. Notably, PePE exhibited specific efficacy against Salmonella enterica adhesion,
attributed mostly to its gallic acid content, whereas PoPE reduced S. enterica invasion in Caco-2 cells. The extracts
supported the prevalence of non-pathogenic and commensal strains of intestinal and skin surfaces,
selectively reducing pathogenic adhesion. The extracts mitigated the oxidative stress, enhanced the barrier
function, and modulated the pro-inflammatory cytokines in LPS-challenged cells. GrPE, rich in anthocyanins, and
PePE were found to mediate their protective effects through Nrf2 activation, while PoPE exerted multifaceted
actions independent of Nrf2.
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Keywords/Subjects: Food by-products Polyphenols Adhesion inhibition Anti-inflammatory activity Nrf2 |
Type of document: info:eu-repo/semantics/article |
Access rights: info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI: https://doi.org/10.1016/j.phymed.2024.156020 |
Appears in Collections: Artículos Agroquímica y Medio Ambiente
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