Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/31134

Plasma ACE2 species are differentially altered in COVID-19 patients

Title:
Plasma ACE2 species are differentially altered in COVID-19 patients
Authors:
García Ayllón, María Salud
Moreno Pérez, Óscar
García Arriaza, Juan
Ramos Rincón, José Manuel
Cortés Gómez, María Ángeles
Brinkmalm, Gunnar
Andrés, Mariano  
León Ramírez, José Manuel
Boix Martínez, Vicente
Gil, Joan
Zetterberg, Henrik
Esteban, Mariano
Merino de Lucas, Esperanza
Editor:
Wiley [Commercial Publisher]
Department:
Instituto de Neurociencias
Departamentos de la UMH::Medicina Clínica
Issue Date:
2021-08
URI:
https://hdl.handle.net/11000/31134
Abstract:
Studies are needed to identify useful biomarkers to assess the severity and prognosis of COVID-19 disease, caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) virus. Here, we examine the levels of various plasma species of the SARS-CoV-2 host receptor, the angiotensin-converting enzyme 2 (ACE2), in patients at different phases of the infection. Human plasma ACE2 species were characterized by immunoprecipitation and western blotting employing antibodies against the ectodomain and the C-terminal domain, using a recombinant human ACE2 protein as control. In addition, changes in the cleaved and full-length ACE2 species were also examined in serum samples derived from humanized K18-hACE2 mice challenged with a lethal dose of SARS-CoV-2. ACE2 immunoreactivity was present in human plasma as several molecular mass species that probably comprise truncated (70 and 75 kDa) and full-length forms (95, 100, 130, and 170 kDa). COVID-19 patients in the acute phase of infection (n = 46) had significantly decreased levels of ACE2 full-length species, while a truncated 70-kDa form was marginally higher compared with non-disease controls (n = 26). Levels of ACE2 full-length species were in the normal range in patients after a recovery period with an interval of 58-70 days (n = 29), while the 70-kDa species decreased. Levels of the truncated ACE2 species served to discriminate between individuals infected by SARS-CoV-2 and those infected with influenza A virus (n = 17). In conclusion, specific plasma ACE2 species are altered in patients with COVID-19 and these changes normalize during the recovery phase. Alterations in ACE2 species following SARS-CoV-2 infection warrant further investigation regarding their potential usefulness as biomarkers for the disease process and to asses efficacy during vaccination.
Keywords/Subjects:
ACE2
biomarker
COVID- 19
plasma
SARS- CoV- 2
Knowledge area:
CDU: Ciencias aplicadas: Medicina: Patología. Medicina clínica. Oncología
Type of document:
application/pdf
Access rights:
info:eu-repo/semantics/openAccess
DOI:
https://doi.org/10.1096/fj.202100051R
Appears in Collections:
Artículos Medicina Clínica



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