Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/30835

Fibroblasts and osteoblasts behavior after contact with different titanium surfaces used as implant abutment: An in vitro experimental study


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Title:
Fibroblasts and osteoblasts behavior after contact with different titanium surfaces used as implant abutment: An in vitro experimental study
Authors:
Cavalcanti de Lima, José Henrique
Robbs , Patricia Cristina  
Mavropoulos, Elena  
De Aza, Piedad  
da Costa, Eleani Maria
SCARANO, Antonio
Prados Frutos, Juan Carlos
Oliveira Fernandes, Gustavo Vicentis
Gehrke, Sergio Alexandre
Editor:
Elsevier
Department:
Departamentos de la UMH::Ciencia de Materiales, Óptica y Tecnología Electrónica
Issue Date:
2024-01
URI:
https://hdl.handle.net/11000/30835
Abstract:
Background: The goal of this in vitro study was to compare three different surfaces: two types of implant surfaces commercially available ([a] smooth/machined and [b] acid-treated surface) versus (c) anodized surface. Discs were manufactured with commercially pure titanium (CP) grade IV, which were subsequently analyzed by scanning microscopy and fibroblastic and osteoblastic cell cultures. Methods: Ninety-nine discs (5 ×2 mm) were manufactured in titanium grade IV and received different surface treatments: (i) Mach group: machined; (ii) AA group: double acid etch; and (iii) AN group: anodizing treatment. Three discs from each group were analyzed by Scanning Electron Microscopy (SEM) to obtain surface topography images and qualitatively analyzed by EDS. Balb/c 3T3 fibroblasts and pre-osteoblastic cells (MC3T3-E1 lineage) were used to investigate each group’s biological response (n =10/cellular type). The data were compared statistically using the ANOVA one-way test, considered as a statistically significant difference p <0.05. Results: The AA group had numerous micropores with diameters between 5 and 10 μm, while nanopores between 1 and 5 nm were measured in the AN group. The EDX spectrum showed a high titanium concentration in all the analyzed samples. The contact angle and wetting tension were higher in the AA, whereas similar results were observed for the other groups. A lower result was observed for base width in the AA, which was higher in the other two groups. The AN showed the best values in the fibroblast cells, followed by Mach and AA; whereas, in the culture of the MC3T3 cells, the result was precisely the opposite (AA >Mach >AN). There was similar behavior for cell adhesion for the test groups (Mach and AN), with greater adhesion of Balb/c 3T3 fibroblasts
Keywords/Subjects:
Surface properties
Titanium
Osteoblasts
Fibroblasts
In vitro
Cell adhesion
Knowledge area:
CDU: Ciencias aplicadas: Cuestiones generales de las ciencias aplicadas
Type of document:
application/pdf
Access rights:
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI:
https://doi.org/10.1016/j.heliyon.2024.e25038
Appears in Collections:
Artículos CIENCIA DE LOS MATERIALES ÓPTICA Y TECNOLOGÍA ELECTRÓNICA



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