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Joint nociceptor nerve activity and pain in an animal model of acute gout and its modulation by intra-articular hyaluronan
Title: Joint nociceptor nerve activity and pain in an animal model of acute gout and its modulation by intra-articular hyaluronan |
Authors: Marcotti, Aida Miralles, Ana Domínguez, Eduardo Pascual Gómez, Eliseo Ángel Gomis García, Ana María Belmonte Martínez, Carlos De la Peña García, Elvira ![](/image/ORCID.jpg) |
Editor: Lippincott, Williams & Wilkins [Commercial Publisher] |
Department: Departamentos de la UMH::Medicina Clínica Instituto de Neurociencias Departamentos de la UMH::Fisiología |
Issue Date: 2018-04 |
URI: https://hdl.handle.net/11000/30713 |
Abstract:
The mechanisms whereby deposition of monosodium urate (MSU) crystals in gout activates nociceptors to induce joint pain are incompletely understood. We tried to reproduce the signs of painful gouty arthritis, injecting into the knee joint of rats suspensions containing amorphous or triclinic, needle MSU crystals. The magnitude of MSU-induced inflammation and pain behavior signs were correlated with the changes in firing frequency of spontaneous and movement-evoked nerve impulse activity recorded in single knee
joint nociceptor saphenous nerve fibers. Joint swelling, mechanical and cold allodynia, and hyperalgesia appeared 3 hours after joint injection of MSU crystals. In parallel, spontaneous and movement-evoked joint nociceptor impulse activity raised significantly.
Solutions containing amorphous or needle-shaped MSU crystals had similar inflammatory and electrophysiological effects. Intraarticular injection of hyaluronan (HA, Synvisc), a high-MW glycosaminoglycan present in the synovial fluid with analgesic effects in osteoarthritis, significantly reduced MSU-induced behavioral signs of pain and decreased the enhanced joint nociceptor activity. Our results support the interpretation that pain and nociceptor activation are not triggered by direct mechanical stimulation of
nociceptors by MSU crystals, but are primarily caused by the release of excitatory mediators by inflammatory cells activated by MSU crystals. Intra-articular HA decreased behavioral and electrophysiological signs of pain, possibly through its viscoelastic filtering effect on the mechanical forces acting over sensitized joint sensory endings and probably also by a direct interaction of HA molecules with the transducing channels expressed in joint nociceptor terminals.
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Keywords/Subjects: Gout Arthritis Hyaluronan Nociceptors Knee-joint inflammation TRP channels |
Type of document: application/pdf |
Access rights: info:eu-repo/semantics/openAccess |
DOI: 10.1097/j.pain.0000000000001137 |
Appears in Collections: Artículos Fisiología
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