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Alternative methods to animal experimentation for testing developmental toxicity


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Title:
Alternative methods to animal experimentation for testing developmental toxicity
Authors:
Pamies, David  
Estevan Martinez, Carmen  
Vilanova, Eugenio
SOGORB, MIGUEL A
Editor:
Elsevier
Department:
Departamentos de la UMH::Biología Aplicada
Issue Date:
2022-02
URI:
https://hdl.handle.net/11000/30667
Abstract:
The available alternative methods for testing developmental toxicity comprise either cellular models or whole embryos of rodents, fish, or amphibian. The simplest cellular models consider the use of human or animal embryonic stem cells (embryonic or of induced pluripotency) under differentiation and one of the most widely used endpoints in these methods is the alterations in differentiated beating cardiomyocytes, although determination of other molecular markers is also extended. More complex cellular models consider the use of cocultures or 3D cultures or the so-called organoids. These models mimic the physiological environment in a much better way than the simple monolayer cultures. The use of whole embryos allows the determination of which teratogenic effects are expectable after exposure to developmental toxicants, which is one of the main disadvantages of the cellular methods. The appropriate assessment of chemical safety for development needs of a battery of alternative methods applied. Integrated Approaches to Testing and Assessment (IATA) would allow in the close future to perform safe and reliable assessment of developmental toxicity based on alternative methods.
Keywords/Subjects:
3D cellular culture
Developmental toxicity
Embryonic stem cell
IATA
Induced pluripotent stem cell
Neurodevelopmental toxicity
Organoid
Testing battery
Knowledge area:
CDU: Ciencias puras y naturales: Biología: Biología general y teórica
Type of document:
application/pdf
Access rights:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI:
https://doi.org/10.1016/B978-0-323-89773-0.00007-2
Appears in Collections:
Libros /Capítulos de Libros- Biología Aplicada



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