Please use this identifier to cite or link to this item:
https://hdl.handle.net/11000/30665
Albumin, the responsible protein of the Cu2+-dependent hydrolysis of O-hexyl O-2,5-dichlorophenyl phosphoramidate (HDCP) by chicken serum "antagonistic stereoselectivity"
View/Open: 2018 Monroy et al FCT 120 523-527 Albumin the reponsible protein ...pdf
765,62 kB
Adobe PDF
Share:
This resource is restricted
Title: Albumin, the responsible protein of the Cu2+-dependent hydrolysis of O-hexyl O-2,5-dichlorophenyl phosphoramidate (HDCP) by chicken serum "antagonistic stereoselectivity" |
Authors: Monroy Noyola, Antonio ![](/image/ORCID.jpg) SOGORB, MIGUEL A ![](/image/ORCID.jpg) Vilanova, Eugenio |
Editor: Elsevier |
Department: Departamentos de la UMH::Biología Aplicada |
Issue Date: 2018-07 |
URI: https://hdl.handle.net/11000/30665 |
Abstract:
O-hexyl O-2,5-dichlorophenyl phosphoramidate (HDCP) is a chiral analogous compound of the methamidophos insecticide that induces delayed neuropathy, and the R-(+)-HDCP enantiomer is an inhibitor of neuropathy target esterase (NTE). This enantiomer is not hydrolized by Ca2+-dependent phosphotriesterases in mammal tissues. Our group had reported R-(+)-HDCP hydrolysis in chicken serum enhanced by 30–250 μM copper in ex vivo assays, which we call "antagonistic stereoselectivity". We checked the hypothesis of the role of cupper binding proteins. Two hundred micrograms of human serum ceruloplasmine or horse kidney methallotionein in 1 mL containing 400 μM HDCP for 60 min showed no significant Cu2+-dependent hydrolysis. However under the same conditions, 10 μL of chicken serum or 10 μL of buffer containing 216 μg of chicken serum albumin (CSA) (amount of albumin content in this serum volume) with 100 μM Cu2+ showed the same stereoselectivity and similar levels to the Cu2+-dependent R-(+)-HDCP hydrolysis. About 75% of R-(+)-HDCP were hydrolyzed after 120 min in the presence of 100 μM Cu2+ (inhibited by 5 mM EDTA). No effects was observed by divalent cations Cu2+, Zn2+, Fe2+, Ca2+, Mn2+ and Mg2+. These results confirm that albumin is the protein responsible for "antagonistic stereoselectivity" observed in chicken seru
|
Keywords/Subjects: Albumin Copper Hydrolysis Stereospecificity Phosphotriesterases Organophosphorus A-esterases |
Knowledge area: CDU: Ciencias puras y naturales: Biología: Biología general y teórica |
Type of document: application/pdf |
Access rights: info:eu-repo/semantics/closedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI: https://doi.org/10.1016/j.fct.2018.07.047 |
Appears in Collections: Artículos Biología Aplicada
|
???jsp.display-item.text9???