Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/6986
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dc.contributor.authorChico, Verónica-
dc.contributor.authorSalvador Mira, María Elizabhet-
dc.contributor.authorNombela Díaz, Iván-
dc.contributor.authorPuente Marín, Sara-
dc.contributor.authorCiordia, Sergio-
dc.contributor.authorMena, María Carmen-
dc.contributor.authorPérez, Luis-
dc.contributor.authorColl Morales, Julio-
dc.contributor.authorGuzman, Fanny-
dc.contributor.authorEncinar Hidalgo, José Antonio-
dc.contributor.authorMercado Cerda, Luis Antonio-
dc.contributor.authorOrtega-Villaizan Romo, María del Mar-
dc.date.accessioned2021-01-25T09:21:58Z-
dc.date.available2021-01-25T09:21:58Z-
dc.date.created2019-03-07-
dc.date.issued2021-01-25-
dc.identifier.issn1664-3224-
dc.identifier.urihttp://hdl.handle.net/11000/6986-
dc.description.abstractViral hemorrhagic septicemia virus (VHSV) infection appears to be halted in rainbow trout nucleated red blood cells (RBCs). Diverse mechanisms are thought to be related to the antiviral immune response of rainbow trout RBCs to VHSV. However, the specific rainbow trout RBC proteins that interact directly with VHSV are still unknown. In an attempt to identify VHSV-RBC protein interactions, we characterized the immunoprecipitated (IP) proteome of RBCs exposed to VHSV using an antibody against the N protein of VHSV. The IP proteomic characterization identified 31 proteins by mass spectrometry analysis. Among them, we identified interferon-induced protein with tetratricopeptide repeats 5 (IFIT5), a protein belonging to a family of proteins that are induced after the production of type I interferon. Importantly, IFIT5 has been implicated in the antiviral immune response. We confirmed the participation of IFIT5 in the rainbow trout RBC antiviral response by examining the expression profile of IFIT5 in RBCs after VHSV exposure at transcriptional and protein levels. We detected a correlation between the highest IFIT5 expression levels and the decline in VHSV replication at 6 h post-exposure. In addition, silencing ifit5 resulted in a significant increase in VHSV replication in RBCs. Moreover, an increase in VHSV replication was observed in RBCs when the IFIT5 RNA-binding pocket cavity was modulated by using a natural compound from the SuperNatural II database. We performed a proximity ligation assay and detected a significant increase in positive cells among VHSV-exposed RBCs compared to unexposed RBCs, indicating protein-protein colocalization between IFIT5 and the glycoprotein G of VHSV. In summary, these results suggest a possible role of IFIT5 in the antiviral response of RBCs against VHSV.es
dc.description.sponsorshipThis work was supported by the European Research Council (ERC Starting Grant GA639249).-
dc.description.sponsorshipThe proteomic analysis was performed in the Proteomics Facility of the Spanish National Center for Biotechnology (CNB-CSIC) belonging to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019-
dc.formatapplication/pdfes
dc.format.extent15es
dc.language.isoenges
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectrainbow troutes
dc.subjectFIT5es
dc.subjectred blood cellses
dc.subjecterythrocytees
dc.subjectVHSVes
dc.subjectantiviral immune responsees
dc.subjectmmunoprecipitatees
dc.subjectproteomices
dc.subject.other577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleIFIT5 Participates in the Antiviral Mechanisms of Rainbow Trout Red Blood Cellses
dc.typeinfo:eu-repo/semantics/articlees
dc.contributor.instituteInstituto de Biología Molecular y Celulares
dc.identifier.doi10.3389/fimmu.2019.00613-
dc.relation.publisherversionhttps://doi.org/10.3389/fimmu.2019.00613-
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Instituto de Biología Molecular y Celular


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