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Recognition of peptidoglycan and b-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae

Título :
Recognition of peptidoglycan and b-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae
Autor :
Maestro García-Donas, Beatriz
Novaková, Linda
Hesek, Dusan  
Leyva, Eduardo  
Mobashery, Shahriar  
Sanz, Jesús M.
Branny, Pavel
Editor :
Elsevier
Fecha de publicación:
2010
URI :
https://hdl.handle.net/11000/30961
Resumen :
The eukaryotic-type serine/threonine kinase StkP from Streptococcus pneumoniae is an important signal-transduction element that regulates the expression of numerous pneumococcal genes. We have expressed the extracellular C-terminal domain of StkP kinase (C-StkP), elaborated a three-dimensional structural model and performed a spectroscopical characterization of its structure and stability. Biophysical experiments show that C-StkP binds to synthetic samples of the cell wall peptidoglycan (PGN) and to β-lactam antibiotics, which mimic the terminal portions of the PGN stem peptide. This is the first experimental report on the recognition of a minimal PGN unit by a PASTA-containing kinase, suggesting that non-crosslinked PGN may act as a signal for StkP function and pointing to this protein as an interesting target for β-lactam antibiotics.
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/openAccess
DOI :
https://doi.org/10.1016/j.febslet.2010.12.016
Aparece en las colecciones:
Instituto de Bioingeniería



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