Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/30806

A Suppressor Screen for AGO1 Degradation by the Viral F-Box P0 Protein Uncovers a Role for AGO DUF1785 in sRNA Duplex Unwinding


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Title:
A Suppressor Screen for AGO1 Degradation by the Viral F-Box P0 Protein Uncovers a Role for AGO DUF1785 in sRNA Duplex Unwinding
Authors:
Derrien, Olivier  
Clavel, Marion  
Baumberger, Nicolas
Iki, Taichiro  
Sarazin, Alexis  
Hacquard, Thibaut
Ponce, María Rosa  
Ziegler-Graff, Véronique  
VAUCHERET, HERVE  
Micol, José Luis  
Voinnet, Olivier  
Genschik, Pascal
Editor:
Oxford University Press
Department:
Departamentos de la UMH::Biología Aplicada
Issue Date:
2018-06
URI:
https://hdl.handle.net/11000/30806
Abstract:
In Arabidopsis thaliana, ARGONAUTE1 (AGO1) plays a central role in microRNA (miRNA) and small interfering RNA (siRNA)-mediated silencing and is a key component in antiviral responses. The polerovirus F-box P0 protein triggers AGO1 degradation as a viral counterdefense. Here, we identified a motif in AGO1 that is required for its interaction with the S phase kinase-associated protein1-cullin 1-F-box protein (SCF) P0 (SCFP0) complex and subsequent degradation. The AGO1 P0 degron is conserved and confers P0-mediated degradation to other AGO proteins. Interestingly, the degron motif is localized in the DUF1785 domain of AGO1, in which a single point mutation (ago1-57, obtained by forward genetic screening) compromises recognition by SCFP0. Recapitulating formation of the RNA-induced silencing complex in a cell-free system revealed that this mutation impairs RNA unwinding, leading to stalled forms of AGO1 still bound to double-stranded RNAs. In vivo, the DUF1785 is required for unwinding perfectly matched siRNA duplexes, but is mostly dispensable for unwinding imperfectly matched miRNA duplexes. Consequently, its mutation nearly abolishes phased siRNA production and sense transgene posttranscriptional gene silencing. Overall, our work sheds new light on the mode of AGO1 recognition by P0 and the in vivo function of DUF1785 in RNA silencing.
Knowledge area:
CDU: Ciencias puras y naturales: Biología
Type of document:
application/pdf
Access rights:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI:
https://doi.org/10.1105/tpc.18.00111
Appears in Collections:
Artículos Biología Aplicada



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