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Analysis of the Arabidopsis venosa4-0 mutant supports the role of VENOSA4 in dNTP metabolism


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Título :
Analysis of the Arabidopsis venosa4-0 mutant supports the role of VENOSA4 in dNTP metabolism
Autor :
Sarmiento, Raquel  
Fontcuberta-Cervera, Sara  
González-Bayón, Rebeca
Hannah, Matthew A.
Álvarez-Martínez, Francisco Javier  
Barrajón-Catalán, Enrique
Micol, Vicente
Quesada, Víctor  
Ponce, María Rosa  
Micol, José Luis  
Editor :
Elsevier
Departamento:
Departamentos de la UMH::Biología Aplicada
Fecha de publicación:
2023-08-09
URI :
https://hdl.handle.net/11000/30803
Resumen :
Human Sterile alpha motif and histidine-aspartate domain containing protein 1 (SAMHD1) functions as a dNTPase to maintain dNTP pool balance. In eukaryotes, the limiting step in de novo dNTP biosynthesis is catalyzed by RIBONUCLEOTIDE REDUCTASE (RNR). In Arabidopsis, the RNR1 subunit of RNR is encoded by CRINKLED LEAVES 8 (CLS8), and RNR2 by three paralogous genes, including TSO MEANING 'UGLY' IN CHINESE 2 (TSO2). In plants, DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS 1 (DOV1) catalyzes the first step of the de novo biosynthesis of purines. Here, to explore the role of VENOSA4 (VEN4), the most likely Arabidopsis ortholog of human SAMHD1, we studied the ven4‐0 point mutation, whose leaf phenotype was stronger than those of its insertional alleles. Structural predictions suggested that the E249L substitution in the mutated VEN4-0 protein rigidifies its 3D structure. The morphological phenotypes of the ven4, cls8, and dov1 single mutants were similar, and those of the ven4 tso2 and ven4 dov1 double mutants were synergistic. The ven4‐0 mutant had reduced levels of four amino acids related to dNTP biosynthesis, including glutamine and glycine, which are precursors in the de novo purine biosynthesis. Our results reveal high functional conservation between VEN4 and SAMHD1 in dNTP metabolism.
Palabras clave/Materias:
dNTP metabolism
Arabidopsis
VENOSA4 gene
ven4-0 mutant
SAMHD1 ortholog
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI :
https://doi.org/10.1016/j.plantsci.2023.111819
Aparece en las colecciones:
Artículos Biología Aplicada



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.