Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/30731
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dc.contributor.authorEsquembre, Rocío-
dc.contributor.authorRenart, María Lourdes-
dc.contributor.authorPoveda, José Antonio-
dc.contributor.authorMateo , C. Reyes-
dc.contributor.otherDepartamentos de la UMH::Agroquímica y Medio Ambientees_ES
dc.date.accessioned2024-01-26T10:41:07Z-
dc.date.available2024-01-26T10:41:07Z-
dc.date.created2022-10-05-
dc.identifier.citationMolecules 2022, 27, 6658es_ES
dc.identifier.issn1420-3049-
dc.identifier.urihttps://hdl.handle.net/11000/30731-
dc.description.abstractThe nanostructuration of solid matrices with lipid nanoparticles containing membrane proteins is a promising tool for the development of high-throughput screening devices. Here, sol-gel silica-derived nanocomposites loaded with liposome-reconstituted KcsA, a prokaryotic potassium channel, have been synthesized. The conformational and functional stability of these lipid nanoparticles before and after sol-gel immobilization have been characterized by using dynamic light scattering, and steady-state and time-resolved fluorescence spectroscopy methods. The lipid-reconstituted KcsA channel entrapped in the sol-gel matrix retained the conformational and stability changes induced by the presence of blocking or permeant cations in the buffer (associated with the conformation of the selectivity filter) or by a drop in the pH (associated with the opening of the activation gate of the protein). Hence, these results indicate that this novel device has the potential to be used as a screening platform to test new modulating drugs of potassium channels.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent16es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectimmobilizationes_ES
dc.subjection channeles_ES
dc.subjectnanostructured materialses_ES
dc.subjectproteoliposomeses_ES
dc.subjectsilica-matriceses_ES
dc.subject.classificationQuímica Físicaes_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::54 - Químicaes_ES
dc.titleSilica/Proteoliposomal Nanocomposite as a Potential Platform for Ion Channel Studieses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.3390/molecules27196658es_ES
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Artículos Agroquímica y Medio Ambiente


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