Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/11000/31153

In vitro analysis of the factors contributing to the antiviral state induced by a plasmid encoding the viral haemorrhagic septicaemia virus glycoprotein G in transfected trout cells


no-thumbnailVer/Abrir:

 Vaccine-2011-main (1).pdf



651,98 kB
Adobe PDF
Compartir:

Este recurso está restringido

Título :
In vitro analysis of the factors contributing to the antiviral state induced by a plasmid encoding the viral haemorrhagic septicaemia virus glycoprotein G in transfected trout cells
Autor :
Ortega-Villaizan, Maria del Mar  
CHICO GRAS, VERONICA  
Martinez-Lopez, A.
Falco, Antonio  
Perez, L.
Coll, J.M.
Estepa, E.
Editor :
Elsevier
Fecha de publicación:
2010-11
URI :
https://hdl.handle.net/11000/31153
Resumen :
We have found out that transfection of the RTG-2 cell line with the viral haemorrhagic septicaemia virus (VHSV) glycoprotein G (GVHSV)-coding plasmid induces an anti-VHSV state, similar to that induced by poly I:C. Taking the advantage of the constitutive expression of toll-like receptor 9 gene (tlr9) in RTG-2 cells, we have investigated whether this antiviral state was induced by the cytosine-phosphodiester-guanine (CpG) motifs present in the plasmid DNA, by the endogenous expression of GVHSV protein or by both elements. For that, we have analysed the expression profile of the rainbow trout tlr9 and several genes related to TLR9-mediated immune response in the absence or presence of a lysosomotropic drug that specifically blocks TLR9–CpG DNA interaction. The results suggested that the high levels of cell protection conferred by a plasmid encoding GVHSV gene are due to GVHSV rather than to the CpG motifs within plasmid DNA. Therefore, plasmid DNA might not play a key role in the immune response elicited by DNA vaccines or perhaps other receptors instead TLR9 could be implicated in CpG motifs recognition and signalling. In addition, since RTG-2 cells express tlr9 gene, this cell line could be a good tool for screening TLR9 agonists, such as the immunomodulatory oligonucleotides (IMOs), as fish DNA vaccine adjuvants.
Palabras clave/Materias:
Innate immunity
Toll-like receptor
TLR9
DNA vaccine
Viral glycoproteins
VHSV
Rhabdovirus
Rainbow trout
RTG-2 cell line
TNFα
IL1β
IRF3
IRF7
Mx
Unmethylated CpG motifs
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología: Biología general y teórica
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI :
https://doi.org/10.1016/j.vaccine.2010.11.021
Aparece en las colecciones:
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.