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Pepscan Mapping of Viral Hemorrhagic Septicemia Virus Glycoprotein G Major Lineal Determinants Implicated in Triggering Host Cell Antiviral Responses Mediated by Type I Interferon


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Título :
Pepscan Mapping of Viral Hemorrhagic Septicemia Virus Glycoprotein G Major Lineal Determinants Implicated in Triggering Host Cell Antiviral Responses Mediated by Type I Interferon
Autor :
CHICO GRAS, VERONICA  
Martinez-Lopez, A.
Ortega-Villaizan, Maria del Mar  
Falco, Antonio  
Perez, L.
Coll, J. M.
Estepa, A.
Editor :
American Society for Microbiology
Fecha de publicación:
2010-07
URI :
https://hdl.handle.net/11000/31147
Resumen :
Surface glycoproteins of enveloped virus are potent elicitors of type I interferon (IFN)-mediated antiviral responses in a way that may be independent of the well-studied genome-mediated route. However, the viral glycoprotein determinants responsible for initiating the IFN response remain unidentified. In this study, we have used a collection of 60 synthetic 20-mer overlapping peptides (pepscan) spanning the full length of glycoprotein G (gpG) of viral hemorrhagic septicemia virus (VHSV) to investigate what regions of this protein are implicated in triggering the type I IFN-associated immune responses. Briefly, two regions with ability to increase severalfold the basal expression level of the IFN-stimulated mx gene and to restrict the spread of virus among responder cells were mapped to amino acid residues 280 to 310 and 340 to 370 of the gpG protein of VHSV. In addition, the results obtained suggest that an interaction between VHSV gpG and integrins might trigger the host IFN-mediated antiviral response after VHSV infection. Since it is known that type I IFN plays an important role in determining/modulating the protective-antigen-specific immune responses, the identification of viral glycoprotein determinants di-rectly implicated in the type I IFN induction might be of special interest for designing new adjuvants and/or more-efficient and cost-effective viral vaccines as well as for improving our knowledge on how to stimulate the innate immune system.
Palabras clave/Materias:
hemorrhagic septicemia virus
IFN-stimulated mx gene
protein are implicated in triggering the type I IFN-associated immune responses
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología: Biología general y teórica
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI :
https://doi.org/10.1128/jvi.00023-10
Aparece en las colecciones:
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.