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https://hdl.handle.net/11000/25691
Reprogramación celular. Modulando las vías de señalización determinantes del destino neuronal
Title: Reprogramación celular. Modulando las vías de señalización determinantes del destino neuronal |
Authors: Sharko, Vira |
Tutor: Ferrer-Montiel, Antonio  Ferrándiz Huertas, Clotilde |
Editor: Universidad Miguel Hernández de Elche |
Department: Departamentos de la UMH::Bioquímica y Biología Molecular |
Issue Date: 2021-06-22 |
URI: http://hdl.handle.net/11000/25691 |
Abstract:
El presente trabajo se plantea con el objetivo de determinar la efectividad de un protocolo de diferenciación directa, basado en la modulación de la expresión génica y de vías de señalización involucradas en la neurogénesis, para la obtención de un modelo neuronal nociceptivo humano a partir de fib... Ver más
The present work is intended to determine the effectiveness of a direct differentiation protocol, based on the modulation of gene expression and signalling pathways involved in neurogenesis, in order to obtain a human nociceptive neuronal model from neonatal fibroblasts. For this purpose, an experimental design structured in three blocks has been used: the first block is focused on the production of lentivirus for the transduction of fibroblasts with sensory neuronal lineage determining genes, the second block is dedicated to the design and optimization of a differentiation protocol composed by small signalling molecules and neurotrophins, and the third block is directed towards the characterization of the induced cells through morphological, genetic and molecular analysis. The results derived from this approach reveal a series of morphological patterns that evoke the structures adopted by the endogenous neurons during the embryonic development. These changes are supported by the expression levels of specific neuronal markers, which, in addition, can be related to the exposure to certain molecules of the protocol. To sum up, this study allows the obtention of an early nociceptive model, laying the foundations for the investigation of more advanced, electrically and functionally relevant sensory models, for the study of human neurological pain.
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Keywords/Subjects: Dolor neuropático Fibroblastos Neuronas nociceptivas peptidérgicas neonatales |
Knowledge area: CDU: Ciencias puras y naturales: Biología |
Type of document: info:eu-repo/semantics/masterThesis |
Access rights: info:eu-repo/semantics/openAccess |
Appears in Collections: TFM-M.U en Biotecnología y Bioingeniería
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