https://hdl.handle.net/11000/521 2026-06-07T08:26:52Z https://hdl.handle.net/11000/40032 Título : Phenolic and triterpenoid composition and inhibition of α-amylase of pistachio kernels (Pistacia vera L.) as affected by rootstock and irrigation treatment Autor : Noguera Artiaga, Luis; Pérez López, David; Burgos Hernández, Armando; Wojdyło, Aneta; Carbonell Barrachina, Ángel A. Resumen : The current water scarcity forces farmers to adopt new irrigation strategies to save water without jeopardizing the fruit yield and quality. In this study, the influence of 3 regulated deficit irrigation (RDI) treatments and 3 rootstocks on the functional quality of pistachios were studied. The functional parameters studied included, polyphenols, triterpenoids, and inhibition of α-amylase. The results showed that P. terebinthus and P. atlantica rootstocks led to pistachio kernels with higher contents of polyphenols and triterpenoids (mainly betulinic acid with 111 and 102 µg g−1, respectively) than pistachios obtained using P. integerrima rootstock (81 µg g−1). On the other hand, the use of moderate RDI (T1 treatment) increased the total content of polyphenols (∼10%), quercetin-O-galloyl-hexoside (∼15%), keampferol-3-O-glucoside (∼19%), and polymeric procyanidins (∼20%), as compared to the control trees, resulting in pistachios with a better functional profile, lower economic cost and with a lesser environmental impact. 2026-06-04T14:13:07Z https://hdl.handle.net/11000/40031 Título : Octopus vulgaris ink extracts exhibit antioxidant, antimutagenic, cytoprotective, antiproliferative, and proapoptotic effects in selected human cancer cell lines Autor : Hernández Zazueta, Martin Samuel; García Romo, Joel Said; Noguera Artiaga, Luis; Luzardo Ocampo, Iván; Carbonell-Barrachina, Ángel Antonio; Taboada Antelo, Pablo; Campos Vega, Rocio; Rosas Burgos, Ema Carina; Burboa Zazueta, María Guadalupe; Ezquerra Brauer, Josafat Marina; Martínez Soto, Juan Manuel; Santacruz Ortega, Hisila del Carmen; Burgos Hernández, Armando Resumen : Cancer is a noncommunicable disease of rising worldwide concern. Marine food products such as Octopusvulgaris ink (OI) could be sources of compounds addressing these concerns. This study aimed to evaluate the antimutagenic,cytoprotective, antiproliferative, proapoptotic, and antioxidant capacity of OI extracts on human cancer cell lines (22Rv1,HeLa, A549). The ARPE-19 cell line was used as a reference human cell line to evaluate the ink’s cytotoxicity. The waterextract exhibited the highest antimutagenic and cytoprotective effect, but the dichloromethane extract (DM) showedthe lowest half lethal concentration against 22Rv1 cells. Structural elucidation of purified DM fractions (F1, F2, F3)identified an unreported compound, N-(2-ozoazepan-3-yl)-pyrrolidine-2-carboxamide (OPC). DM-F2 showed highantiproliferative effect (LC 50 = 27.6 μg/mL), reactive species modulation, early-apoptosis induction (42.9%), and nucleidisruption in 22Rv1 cells. In silico analysis predicted high OPC affinity with Cyclin D1 (−6.70 kcal/mol), suggestingits potential impact on cell cycle arrest. These results highlight the antimutagenic, cytoprotective, and antiproliferativepotential health benefits derived from underutilized marine food products such as OI. Further investigations at in vitro orin vivo levels are required to elucidate mechanisms and health benefits from OI. 2026-06-04T14:07:45Z https://hdl.handle.net/11000/40030 Título : Opinion of Spanish Consumers on Hydrosustainable Pistachios Autor : Noguera Artiaga, Luis; Lipan, Leontina; Vázquez Araújo, L.; Barber, Xavi; Pérez López, David; Carbonell Barrachina, Ángel A. Resumen : Fruits and vegetables cultivated under controlled deficit irrigation (CDI) are called hydrosustainable (hydroSOS) products and have its own personality and are environmentally-friendly. Focus groups helped in classifying key farming, sensory, and health concepts associated with CDI-grown pistachios. Besides, focus groups also helped in stating that a logo was needed for these special foods, and that a hydroSOS index is also essential to certify that the products have been controlled by a control board. Conjoint analysis was used to check which attributes could be helpful in promoting CDI-grown pistachios among Spanish consumers in a 1st step toward the European Union (EU) market. It was clearly proved that the main silo of properties driving the attention of Spanish consumers was that related to health. The most important attributes for pistachios were “product of Spain,” “rich in antioxidant,” and “crunchy”; this finding was clearly related to the popularity of regional foods, the preoccupation of European consumers for their health, and the joy related to the crunchiness of toasted nuts, respectively. The use of these 3 concepts, together with the use of the hydroSOS logo, will be essential to promote hydroSOS pistachios among Spanish and EU consumers. Finally, it is important to highlight that in general Spanish consumers were willing to pay an extra amount of 1.0 euros per kg of hydroSOS pistachios. These earnings will be essential to convince Spanish farmers to implement CDI strategies and have a sustainable and environmental-friendly use of the irrigation water. 2026-06-04T14:03:58Z https://hdl.handle.net/11000/40029 Título : Volatile Composition, Sensory Profile, and Consumers’ Acceptance of Fondillón Autor : Issa-Issa, Hanán; Noguera-Artiaga, Luis; Sendra, Esther; Pérez-López, Antonio J.; Burló, Francisco; Carbonell-Barrachina, Ángel A.; López-Lluch, David Resumen : No scientific information exists on quality attributes of Fondillón, a special naturally sweet wine produced from overripe Monastrell grapes and one of the only six wines that can use its own name according to European Union Regulations. The aim of this study was to analyze the composition (physicochemical and volatile profile) and sensory quality of this special wine. A specific lexicon to describe wines under the Alicante PDO was developed, using 28 attributes (11 flavor notes, 3 visual, 2 global, and 12 defects). Forty volatile compounds were isolated, and esters were the main chemical family of volatile compounds of Fondillón (∼70%), followed by alcohols (∼20%). Furthermore, two volatile compounds (TDN and vitispirane) were positively correlated with the age of the Fondillón samples, under the specific working conditions used in this study. According to a sensory study, this wine was appreciated by Spanish consumers as having intense fruity notes, high alcohol content, and some bitter and balsamic notes; however, further research is needed to identify the proper profile of Fondillón consumers and their buying and acceptance drivers. 2026-06-04T14:01:03Z https://hdl.handle.net/11000/40028 Título : Chemical composition and cytotoxic potency of essential oil from Seseli petraeum M. Bieb. (Apiaceae) Autor : Önder, Alev; Çınar Koç, Ahsen Sevde; Bakar Ateş, Filiz; Noguera Artiaga, Luis; Carbonell Barrachina, Angel A. Resumen : The present study aimed to investigate the chemical composition and in vitro cytotoxic activity of the fruit essential oil of Seseli petraeum M. Bieb. growing in the Northern side of Anatolia. The Seseli petraeum essential oil was obtained by hydrodistillation from the fruits and analyzed by GC/MS to determine its chemical composition. The major components have been determined as carotol (17.25%), γ-terpinene (10.73%), β-farnesene (8.50%), p-cymene (7.93%), germacrene-D (7.65%), and sabinene (7.31%). The cytotoxic activity of the essential oil was evaluated by MTT assay in vitro on cell proliferation with MCF-7 (Human breast adenocarcinoma) and A549 (Human lung carcinoma) cells. The results showed that the essential oil has potent cytotoxicity against treated cancer cells, whereas it was more cytotoxic in MCF-7 cells (IC50=390.38 μg/mL). The results demonstrated that S. petraeum essential oil has a marked cytotoxic effect against treated cancer cells. Although this effect is likely to be caused by carotol owing to a major component of the oil, further designed studies are necessary due to the possibility of activity, and maybe it occurred due to the synergistic effects of the oil components. The findings of this study may promote the use of the species for pharmaceutical purposes. 2026-06-04T13:56:06Z https://hdl.handle.net/11000/40027 Título : Understanding consumer perception of a new product including apple pomace: The role of health, sustainability, and culinary engagement Autor : Kita, A.; B. Rune, C.J.; Giacalone, D.; Noguera-Artiaga, L.; Carbonell-Barrachina, Á.A.; Nemś, A.; Kolniak-Ostek, J.; Michalska-Ciechanowska, A.; Mora, M.; Romeo-Arroyo, E.; Vázquez-Araújo, L. Resumen : The aim of the present study was to determine perception of a new product made with apple pomace including consumers from three different gastronomy cultures: Spain (Mediterranean), Poland (Central Europe), and Denmark (Nordic). The new product was mainly made from potato and apple pomace and had the typical texture of a savory snack (crisp-like), but it was characterized by its apple/sweet taste. Besides consumers from the aforementioned countries, a group of Foodies-consumers from 20 different nationalities participated in the study to determine potential differences on the product perception due to their high culinary knowledge/ingredients exposure. Over 600 consumers participated in the study. To segments consumers into different groups beyond nationality, the demographic questionnaire included the short version of the Food Choice Questionnaire, the Food Neophobia Scale, and questions on Dietary and Sustainability related habits. The hedonic response, as well as the intrinsic properties of the product (sweetness, sourness, saltiness, apple flavor, hardness, and crunchiness) were measured using Just-About-Right scales. Additional questions included assessment on the storytelling of the product, potential applications/consumption moments, and finally, the impact of adding a health (high in fiber) or a sustainable (made using by-products) claim. Analysis revealed variations in demographics across consumer groups, potentially influencing their perception of the new product. Including the question on applications/consumption moments of the new food, as well as gathering free words about the main aspects consumers valued about the food, were useful to better understand its potential in the market and design a proper communication strategy. 2026-06-04T13:46:29Z https://hdl.handle.net/11000/40026 Título : Valorization of transgenic Tomaffron-based products enriched in saffron apocarotenoids Autor : Lobato-Gómez, Maria; Rambla, José L.; Zacarías-García, Jaime; Noguera-Artiaga, Luis; Pérez-Beser, Jose; Orzáez, Diego; Gómez-Gómez, Lourdes; Granell, Antonio Resumen : Saffron spice (Crocus sativus L.) is highly valued for its bioactive apocarotenoids, including crocins, picrocrocin, and safranal, but its commercial production is limited due to labor-intensive cultivation. In this study, Tomaffron, a transgenic tomato-based platform enriched with saffron apocarotenoids, has been processed into different tomato products to assess their suitability as carriers of these valuable apocarotenoids. Their composition, antioxidant capacity, and sensory attributes were analyzed, with particular attention paid to saffron apocarotenoid stability during processing and storage. Tomaffron-processed products retained or increased the levels of saffron apocarotenoids, with crocin accumulation remaining stable during storage. Picrocrocin and safranal levels remained stable or increased, and sensory tests confirmed that these products replicated the saffron-like flavor. This work highlights the potential of Tomaffron for delivering saffron apocarotenoids in stable, ready-to-consume food matrices. These findings support the feasibility of developing functional food prototypes enriched in valuable plant secondary metabolites using metabolic engineering. 2026-06-04T13:41:26Z https://hdl.handle.net/11000/40025 Título : Effect of the herbs used in the formulation of a Spanish herb liqueur, Herbero de la Sierra de Mariola, on its chemical and functional compositions and antioxidant and antimicrobial activities Autor : Issa‑Issa, Hanán; Ivanišová, Eva; Noguera‑Artiaga, Luis; Kántor, Attila; López‑Lluch, David; Kačániová, Miroslava; Szumny, Antoni; Carbonell‑Barrachina, Ángel A. Resumen : Herbero de la Sierra de Mariola is a traditional Spanish herb liqueur which can have up to 15 different plants. The aim of this study was to help in deciding which are the best suited herbs to be used to prepare a liqueur with the highest contents of bioactive compounds and their functional properties. Chemical parameters (FT-IR spectroscopy), total contents of bioactive compounds (polyphenols, flavonoids, and phenolic acids), sensory quality (total content of volatile compounds and aroma intensity), antioxidant activity (DPPH‧ and ABTS+), and antimicrobial activity, and sensory intensity were the parameters studied. In general, the best highest contents of the bioactive compounds and functional activities were found in liqueurs of the Lamiaceae family. An optimized version of this traditional liqueur might contain (i) winter savory, (ii) fennel, (iii) thyme, and (iv) peppermint. This study can be an example of how the scientific knowledge can be used to optimize traditional products, such as an Herbero de la Sierra de Mariola, and why not also to support the advantages of these products to establish future marketing campaigns. 2026-06-04T10:53:44Z https://hdl.handle.net/11000/40016 Título : Biological activity, antioxidant capacity and volatile profile of enriched Slovak chocolates Autor : Godočiková, Lucia; Ivanišová, Eva; Noguera Artiaga, Luis; Carbonell Barrachina, Ángel A.; Kačániová, Miroslava Resumen : Different kinds and types of chocolates produced in Slovakia with various fruit and nut enrichments were analysed regarding total polyphenols, flavonoids and phenolic acids contents using spectrophotometric methods and their volatile profile was analysed using gas chromatography–mass spectrometry. The method with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing power method (FRAP) were used to measure antioxidant activity. Plain dark chocolate without any enrichment made by a traditional process contained the highest number of total polyphenols and phenolic acids and also had the highest antioxidant activity. Positive correlation was observed between antioxidant activity and total contents of phenolic compounds, flavonoids and phenolic acids. The enrichment with different kinds of fruits did not increase the number of bioactive compounds or antioxidant capacity of chocolates but created more attractive sensory profiles due to the presence of new volatile compounds. 2026-06-04T08:32:12Z https://hdl.handle.net/11000/40015 Título : Antioxidant, antihemolysis, and retinoprotective potentials of bioactive lipidiccompounds from wild shrimp (Litopenaeus stylirostris) muscle Autor : García Romo, Joel Said; Noguera Artiaga, Luis; Gálvez Iriqui, Alma Carolina; Hernández Zazueta, Martin Samuel; Valenzuela Cota, Daniel Fernando; González Vega, Ricardo Iván; Plascencia Jatomea, Maribel; Burboa Zazueta, María Guadalupe; Sandoval Petris, Edgar; Robles Sánchez, Rosario Maribel; Juárez, Josué; Hernández Martínez, Javier; Santacruz Ortega, Hisila del Carmen; Burgos Hernández, Armando Resumen : The oxidative stress damage on cells is an example of roles in the pathogenesis of differentdegenerative diseases and the search of compounds that can slow this oxidation is continuous.The aim of this work was to obtain bioactive fractions from wild shrimp (Litopenaeus stylirostris)muscle, in order to evaluate their protective capacity and chemo-structurally characterize them.ABTS and DPPH, and FRAP assays suggested that bioactive fractions possess free radical-scavengingcapacity, and reducing power, respectively. An inhibitory effect observed on AAPH-induced hemo-lysis and results from an H2O 2-derived radicals-scavenging assay (retinoprotective) suggest thatthese fractions can exert their protective activity in human cells. UV–Vis, fluorescence, 13C-, 1H-NMR,and ESI-MS studies performed on the most bioactive fraction, suggests that their main componentsare eicosapentaenoic acid, dioctyl phthalate, and a possibly novel indolocarbazole alkaloid deriva-tive. These results suggest that these compounds are good candidates to further investigations aspossible chemoprotective agents. 2026-06-04T08:27:48Z