Por favor, use este identificador para citar o enlazar este ítem:
https://hdl.handle.net/11000/36835
Electrochemical quantification of β-glucosidase activity for inhibitor screening applications
Título : Electrochemical quantification of β-glucosidase activity for inhibitor screening applications |
Autor : Farcas, Catalina  Mateo, C. Reyes  Montilla, Francisco  |
Editor : Elsevier |
Departamento: Departamentos de la UMH::Agroquímica y Medio Ambiente |
Fecha de publicación: 2025-04-04 |
URI : https://hdl.handle.net/11000/36835 |
Resumen :
The measurement of enzymatic activity of glucosidases is essential for its use in various clinical and industrial
applications. Inhibitors of these enzymes are promising candidates as antidiabetic drug. This study presents the
development of an electrochemical device for quantifying β-glucosidase activity using an electrochemical
transduction method. Enzyme activity was evaluated in a citrate buffer solution with p-arbutin (hydroquinoneβ-D-glucopyranoside) as the substrate, employed for the first time in this context. The enzymatic hydrolysis of the
glycosidic bond produced hydroquinone, which was quantified via voltammetric measurements to determine the
hydrolysis rate. To develop a device for the screening of inhibitory effects on glucosidase, this enzyme was
immobilized into silica matrix and its activity was evaluated. Entrapped enzyme shows partial retention of its
catalytic function that can be measured by means of the electrochemical transduction. The inhibitory effects of
various sugars and acarbose (a commonly used antidiabetic drug) were investigated using the sensor with the
immobilized enzyme. Inhibition rates detected ranged from 87 % to 13 % for the sugars and 17 % for acarbose.
|
Palabras clave/Materias: Electrochemical biosensor В-Glucosidase P-Arbutin Voltammetric detection Acarbose Enzyme inhibition |
Área de conocimiento : CDU: Ciencias puras y naturales |
Tipo de documento : info:eu-repo/semantics/article |
Derechos de acceso: info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI : https://doi.org/10.1016/j.bioelechem.2025.108981 |
Publicado en: Bioelectrochemistry. 2025, 165: 108981 |
Aparece en las colecciones: Artículos Agroquímica y Medio Ambiente
|
La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.