Título : Formation of Complexes between the Conjugated Polyelectrolyte Poly{[9,9-bis(6′ N,N,Ntrimethylammonium)hexyl]fluorene-phenylene} Bromide (HTMA-PFP) and Human Serum Albumin |
Autor : Martínez-Tome, Maria Jose Esquembre, Rocio Mallavia, Ricardo  C. Reyes, Mateo |
Editor : American Chemical Society |
Departamento: Departamentos de la UMH::Agroquímica y Medio Ambiente |
Fecha de publicación: 2010 |
URI : https://hdl.handle.net/11000/36834 |
Resumen :
The interaction between the conjugated polyelectrolyte poly{[9,9-bis(6′-N,N,N-trimethylammonium)hexyl]fluorenephenylene} bromide (HTMA-PFP) and human serum albumin (HSA) has been investigated from changes observed
in both the spectroscopic properties of HTMA-PFP and the intrinsic fluorescence of HSA. Absorption and
fluorescence spectra of HTMA-PFP suggest that HTMA-PFP and HSA form polymer-protein complexes due to
electrostatic interactions between the cationic side chains of HTMA-PFP and the negatively charged surface of
the protein. Interaction between both macromolecules induces an increase in the fluorescence signal of HTMAPFP, which suggests that hydrophobic forces also contribute to the polymer-protein complex stabilization. In
addition, this interaction causes a decrease in the HSA fluorescence, partially due to static quenching and energy
transfer between both macromolecules. Effects of HTMA-PFP on the thermal stability and protein conformation
were explored from CD experiments. Results indicate that as polymer is added it binds to HSA and initiates
unfolding. This unfolding process induces HTMA-PFP chains to become more extended, disrupting backbone
interactions and increasing polymer fluorescence intensity.
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Área de conocimiento : CDU: Ciencias puras y naturales |
Tipo de documento : info:eu-repo/semantics/article |
Derechos de acceso: info:eu-repo/semantics/closedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI : https://doi.org/10.1021/bm100123t |
Publicado en: Biomacromolecules 2010, 11, 1494–1501 |
Aparece en las colecciones: Artículos Agroquímica y Medio Ambiente
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