Título : Twenty Years of Tomato Breeding at EPSO-UMH: Transfer Resistance from Wild Types to Local Landraces—From the First Molecular Markers to Genotyping by Sequencing (GBS) |
Autor : García Martínez, Santiago  Carbonell, Pedro  Alonso, Aranzazu Grau Sánchez, Adrián  Salinas, Juan Francisco Ruíz, Juan José  |
Editor : MDPI |
Departamento: Departamentos de la UMH::Biología Aplicada |
Fecha de publicación: 2018 |
URI : https://hdl.handle.net/11000/36016 |
Resumen :
In 1998, the plant breeding team at the School of Engineering of Orihuela (EPSO), part
of the Miguel Hernández University (UMH) in Elche, commenced a tomato breeding program.
Marker-assisted selection and backcrossing were used to simultaneously introduce three genes
(Tm-2a, Ty-1, and Sw-5) that confer resistance to relevant viruses, such as tomato mosaic virus (ToMV),
tomato yellow curl virus (TYLCV), and tomato spotted wilt virus (TSWV), to traditional varieties
of local tomatoes, specifically the “Muchamiel” and the “De la pera” types. After each backcross,
cleaved amplified polymorphic sequence (CAPS) molecular markers were used to select the plants
with the resistance genes of interest. A previously described marker was used for TSWV, and new
markers were designed for ToMV, and TYLCV using available sequences in the National Center for
Biotechnology Information (NCBI) database. In parallel to the breeding program, several molecular
markers—Sequence Related Amplified Polymorphism (SRAP), Simple Sequence Repeats (SSRs),
Amplified Fragment Length Polymorphisms (AFLPs), Single Nucleotide Polymorphisms (SNPs),
and (GATA)4 probes—were used to study genetic variability, and to identify a collection of Spanish
and Italian traditional tomato varieties. The results showed a limited genetic variability among
cultivated tomato varieties. The breeding lines Muchamiel UMH 1200, and De la pera 1203 (both with
homozygous resistance to the three viruses) were the first new varieties that were obtained. They were
included in the Register of Protected Plant Varieties in 2013. Lines without a resistance to TYLCV were
also developed, and protected in 2017. We have begun to use SNP massive genotyping for studies
of genetic association, and for selecting plants with the Ty-1 gene with less linkage drag. Molecular
markers have been extremely useful in identifying the different steps of the tomato breeding program
at EPSO-UMH.
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Palabras clave/Materias: De la pera ToMV TYLCV TSWV |
Tipo de documento : info:eu-repo/semantics/article |
Derechos de acceso: info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI : https://doi.org/10.3390/d10010012 |
Aparece en las colecciones: Artículos Biología Aplicada
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