Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/34432
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dc.contributor.authorEstevan Martinez, Carmen-
dc.contributor.authorSOGORB, MIGUEL A-
dc.contributor.authorPamies, David-
dc.contributor.authorde Lapuente, Joaquin-
dc.contributor.authorJorge, Estévez-
dc.contributor.authorVilanova, Eugenio-
dc.contributor.otherDepartamentos de la UMH::Biología Aplicadaes_ES
dc.date.accessioned2025-01-11T17:07:17Z-
dc.date.available2025-01-11T17:07:17Z-
dc.date.created2014-
dc.identifier.citationToxicology Letterses_ES
dc.identifier.issn1879-3169-
dc.identifier.urihttps://hdl.handle.net/11000/34432-
dc.description.abstractThe main available alternatives for testing embryotoxicity are cellular tests with stem cells and in vitro- ex vivo tests with embryos. In cellular tests, the most developed alternative is the embryonic stem cell test, while the most developed tests involving embryos are the zebrafish and whole embryo culture test. They are technically more complex than cellular tests, but offer the advantage of determining the expectable phenotypic alteration caused by the exposure.Many efforts are currently beingmade, basically through proteomic and genomic approaches, in order to obtain improvements in predictivity of these tests. Development is a very complex process, and it is highly unlikely that a single alternative test can yield satisfactory performance with all types of chemicals. We propose a step-wise approach where model complexity, and consequently technical skills and economical costs, gradually increase if needed. The first level would be run short cellular assays to detect effects in early differentiation stages. The second level would involve longer cellular embryotoxicity tests to search embryotoxicants that have an effect on late differentiation stages. The third stage would consider tests with embryos because they allow the determination of hazards based on molecular and morphological alterations, and not only on differentiating cells.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent12es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.ispartofseries230es_ES
dc.relation.ispartofseries2es_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEmbryotoxicityes_ES
dc.subjectTeratogenicityes_ES
dc.subjectAlternative testses_ES
dc.subjectEmbryonic stem cell testes_ES
dc.subjectZebrafish testes_ES
dc.subjectWECes_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::573 - Biología general y teóricaes_ES
dc.titleAn integrated approach for detecting embryotoxicity and developmental toxicity of environmental contaminants using in vitro alternative methodses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.toxlet.2014.01.037es_ES
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Artículos Biología Aplicada


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