Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/32325

β2‑adrenergic receptor functionality and genotype in two different models of chronic inflammatory disease: Liver cirrhosis and osteoarthritis

Title:
β2‑adrenergic receptor functionality and genotype in two different models of chronic inflammatory disease: Liver cirrhosis and osteoarthritis
Authors:
ROCA, REYES
ESTEBAN, PABLO
Zapater, Pedro  
Inda, María del Mar
CONTE, LUCIA  
Gómez-Escolar, Laura  
MARTÍNEZ, HELENA
Horga, José Francisco  
PALAZON, JOSÉ M.
Peiró, Ana  
Editor:
Spandidos publications
Department:
Departamentos de la UMH::Farmacología, Pediatría y Química Orgánica
Issue Date:
2018-06
URI:
https://hdl.handle.net/11000/32325
Abstract:
The present study was designed to investigate the functional status of β2 adrenoceptors (β2AR) in two models of chronic inflammatory disease: liver cirrhosis (LC) and osteoarthritis (OA). The β2AR gene contains three single nucleotide polymorphisms at amino acid positions 16, 27 and 164. The aim of the present study was to investigate the potential influence of lymphocyte β2AR receptor functionality and genotype in LC and OA patients. Blood samples from cirrhotic patients (n=52, hepatic venous pressure gradient 13±4 mmHg, CHILD 7±2 and MELD 11±4 scores), OA patients (n=30, 84% Kellgren‑Lawrence severity 4 grade, 14% knee replacement joint) and healthy volunteers as control group (n=26) were analyzed. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood and basal and isoproterenol induced adenylate cyclase activity (isoproterenol stimulus from 10‑9 to 10‑4 mM), and β2AR allelic variants (rs1042713, rs1042714, rs1800888) were determined. β2AR functionality was decreased in the two different models of chronic inflammatory disease studied, OA (50% vs. control) and LC (85% vs. control). In these patients, the strength of the β2AR response to adrenergic stimulation was very limited. Adrenergic modulation of PBMC function through the β2AR stimulus is decreased in chronic inflammatory processes including LC and OA, suggesting that the adrenergic system may be important in the development of these processes.
Keywords/Subjects:
β2 adrenoceptors
liver cirrhosis
osteoarthritis
polymorphisms
isoproterenol
cAMP
Type of document:
application/pdf
Access rights:
info:eu-repo/semantics/openAccess
DOI:
https://doi.org/10.3892/mmr.2018.8820
Appears in Collections:
Artículos Farmacología, Pediatría y Química Orgánica



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