Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/31176
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dc.contributor.authorDhaouadi, Karima-
dc.contributor.authorRaboudi, Faten-
dc.contributor.authorFunez-Gomez, Lorena-
dc.contributor.authorPamies, David-
dc.contributor.authorEstevan Martinez, Carmen-
dc.contributor.authorHamdaoui, Mohamed-
dc.contributor.authorFattouch, Sami-
dc.contributor.otherDepartamentos de la UMH::Biología Aplicadaes_ES
dc.date.accessioned2024-02-07T07:57:11Z-
dc.date.available2024-02-07T07:57:11Z-
dc.date.created2012-04-21-
dc.identifier.citationFood Analytical Methods, Volume 6, pages 45–53, (2013)es_ES
dc.identifier.issn1936-976X-
dc.identifier.issn1936-9751-
dc.identifier.urihttps://hdl.handle.net/11000/31176-
dc.description.abstractThe traditionally derived syrup of Opuntia ficus-indica fruit is commonly used in homemade confectionery. Herein, the aqueous-acetone extract prepared from the Tunisian O. ficus-indica syrup was investigated. The qualitatively and quantitatively polyphenolic content was analysed using reversed-phase high-performance liquid chromatography–diode array detection (RP-HPLC–DAD) coupled to electrospray ionisation–mass spectrometry (ESI–MS). The extract contained 19.95 ± 2.01 mg phenolics per gram of fresh starting material with isorhamnetin 3-O-robinobioside as the major compound (22.76%). The syrup extract showed strong antioxidant potentials as assessed by both ABTS and DPPH functional methods. It exhibited effective antimicrobial activity, particularly against Staphylococcus aureus and Staphylococcus epidermidis with a minimal bactericide concentration (MBC) of 1.3 mg phenolics/ml. Furthermore, at final concentrations in the range of 41.38–186.25 μg polyphenols/ml, the extract decreased human SH-SY5Y neuroblastoma and 3T3 fibroblast in vitro cell viability in a dose- and time-dependent manner compared to non-treated control cells. The observed effects were significantly (P < 0.05) high against cancer lines. Extract concentrations higher than 106.43 μg/ml reduced cancer cells viability to 50–60% 1–3 h post-treatment. Further in vivo insight studies should emphasise and validate the herein obtained results.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent9es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLC–ESI–MSes_ES
dc.subjectPolyphenolses_ES
dc.subjectSyrupes_ES
dc.subjectAntioxidantes_ES
dc.subjectAntimicrobiales_ES
dc.subjectCancer cellses_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biologíaes_ES
dc.titlePolyphenolic Extract of Barbary-Fig (Opuntia ficus-indica) Syrup: RP–HPLC–ESI–MS Analysis and Determination of Antioxidant, Antimicrobial and Cancer-Cells Cytotoxic Potentialses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.1007/s12161-012-9410-xes_ES
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