Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/31153
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dc.contributor.authorOrtega-Villaizan, Maria del Mar-
dc.contributor.authorCHICO GRAS, VERONICA-
dc.contributor.authorMartinez-Lopez, A.-
dc.contributor.authorFalco, Antonio-
dc.contributor.authorPerez, L.-
dc.contributor.authorColl, J.M.-
dc.contributor.authorEstepa, E.-
dc.date.accessioned2024-02-06T12:14:59Z-
dc.date.available2024-02-06T12:14:59Z-
dc.date.created2010-11-
dc.identifier.citationVaccine Volume 29, Issue 4, 17 January 2011, Pages 737-743es_ES
dc.identifier.issn0264-410X-
dc.identifier.urihttps://hdl.handle.net/11000/31153-
dc.description.abstractWe have found out that transfection of the RTG-2 cell line with the viral haemorrhagic septicaemia virus (VHSV) glycoprotein G (GVHSV)-coding plasmid induces an anti-VHSV state, similar to that induced by poly I:C. Taking the advantage of the constitutive expression of toll-like receptor 9 gene (tlr9) in RTG-2 cells, we have investigated whether this antiviral state was induced by the cytosine-phosphodiester-guanine (CpG) motifs present in the plasmid DNA, by the endogenous expression of GVHSV protein or by both elements. For that, we have analysed the expression profile of the rainbow trout tlr9 and several genes related to TLR9-mediated immune response in the absence or presence of a lysosomotropic drug that specifically blocks TLR9–CpG DNA interaction. The results suggested that the high levels of cell protection conferred by a plasmid encoding GVHSV gene are due to GVHSV rather than to the CpG motifs within plasmid DNA. Therefore, plasmid DNA might not play a key role in the immune response elicited by DNA vaccines or perhaps other receptors instead TLR9 could be implicated in CpG motifs recognition and signalling. In addition, since RTG-2 cells express tlr9 gene, this cell line could be a good tool for screening TLR9 agonists, such as the immunomodulatory oligonucleotides (IMOs), as fish DNA vaccine adjuvants.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent7es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectInnate immunityes_ES
dc.subjectToll-like receptores_ES
dc.subjectTLR9es_ES
dc.subjectDNA vaccinees_ES
dc.subjectViral glycoproteinses_ES
dc.subjectVHSVes_ES
dc.subjectRhabdoviruses_ES
dc.subjectRainbow troutes_ES
dc.subjectRTG-2 cell linees_ES
dc.subjectTNFαes_ES
dc.subjectIL1βes_ES
dc.subjectIRF3es_ES
dc.subjectIRF7es_ES
dc.subjectMxes_ES
dc.subjectUnmethylated CpG motifses_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::573 - Biología general y teóricaes_ES
dc.titleIn vitro analysis of the factors contributing to the antiviral state induced by a plasmid encoding the viral haemorrhagic septicaemia virus glycoprotein G in transfected trout cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.contributor.instituteInstitutos de la UMH::Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elchees_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.vaccine.2010.11.021es_ES
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Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche


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