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dc.contributor.authorCHICO GRAS, VERONICA-
dc.contributor.authorOrtega-Villaizan, Maria del Mar-
dc.contributor.authorFalco, Antonio-
dc.contributor.authorTafalla, C.-
dc.contributor.authorPerez, L.-
dc.contributor.authorColl, J.M.-
dc.contributor.authorEstepa, A.-
dc.identifier.citationVaccine Volume 27, Issue 13, 18 March 2009, Pages 1938-1948es_ES
dc.description.abstractA plasmid DNA encoding the viral hemorrhagic septicaemia virus (VHSV)-G glycoprotein under the control of 5′ upstream sequences (enhancer/promoter sequence plus both non-coding 1st exon and 1st intron sequences) from carp β-actin gene (pAE6-GVHSV) was compared to the vaccine plasmid usually described the gene expression is regulated by the human cytomegalovirus (CMV) immediate-early promoter (pMCV1.4-GVHSV). We observed that these two plasmids produced a markedly different profile in the level and time of expression of the encoded-antigen, and this may have a direct effect upon the intensity and suitability of the in vivo immune response. Thus, fish genetic immunisation assays were carried out to study the immune response of both plasmids. A significantly enhanced specific-antibody response against the viral glycoprotein was found in the fish immunised with pAE6-GVHSV. However, the protective efficacy against VHSV challenge conferred by both plasmids was similar. Later analysis of the transcription profile of a set of representative immune-related genes in the DNA immunized fish suggested that depending on the plasmid-related regulatory sequences controlling its expression, the plasmid might activate distinct patterns of the immune system. All together, the results from this study mainly point out that the selection of a determinate encoded-antigen/vector combination for genetic immunisation is of extraordinary importance in designing optimised DNA vaccines that, when required for inducing protective immune response, could elicit responses biased to antigen-specific antibodies or cytotoxic T cells generation.es_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectVHS viruses_ES
dc.subjectDNA vaccinees_ES
dc.subjectGene expression profilees_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::573 - Biología general y teóricaes_ES
dc.titleThe immunogenicity of viral haemorragic septicaemia rhabdovirus (VHSV) DNA vaccines can depend on plasmid regulatory sequenceses_ES
dc.contributor.instituteInstitutos de la UMH::Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elchees_ES
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Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche


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