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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Maestro García-Donas, Beatriz | - |
dc.contributor.author | Sanz, Jesús M. | - |
dc.date.accessioned | 2024-02-02T09:31:40Z | - |
dc.date.available | 2024-02-02T09:31:40Z | - |
dc.date.created | 2006 | - |
dc.identifier.citation | FEBS Letters . 2007 Feb 6;581(3):375-81 | es_ES |
dc.identifier.issn | 1873-3468 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | https://hdl.handle.net/11000/30953 | - |
dc.description.abstract | We have investigated the stability of the choline-binding module C-LytA against sodium dodecyl sulphate (SDS)-induced unfolding at pH 7.0 and 20 C. A major intermediate with an unfolded N-terminal region accumulates at around 0.75 mM SDS, whereas 2.0 mM SDS was sufficient for a complete unfolding. This might be the first report of a protein being extensively unfolded by submicellar concentrations of SDS, occurring through formation of detergent clusters on the protein surface. All transitions were reversible upon SDS complexation with b-cyclodextrin, allowing the calculation of thermodynamic parameters. A model for the unfolding of C-LytA by SDS is presented and compared to a previous denaturation scheme by guanidine hydrochloride. | es_ES |
dc.format | application/pdf | es_ES |
dc.format.extent | 7 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Choline-binding module | es_ES |
dc.subject | SDS denaturation | es_ES |
dc.subject | Protein stability | es_ES |
dc.subject | Repeat proteins | es_ES |
dc.subject | Affinity tag | es_ES |
dc.title | Extensive unfolding of the C-LytA choline-binding module by submicellar concentrations of sodium dodecyl sulphate | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.contributor.institute | Institutos de la UMH::Instituto de Bioingeniería | es_ES |
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