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Chapter 6: Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique
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Title: Chapter 6: Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique |
Authors: De la Peña García, Elvira Gomis García, Ana María |
Editor: Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. |
Department: Departamentos de la UMH::Fisiología Instituto de Neurociencias |
Issue Date: 2019 |
URI: https://hdl.handle.net/11000/30739 |
Abstract:
Transient receptor potential (TRP) ion channels are involved in a variety of fundamental physiological
processes, and their malfunction produces numerous human diseases. Therefore, these proteins represent a
class of attractive drug targets and a class of important off-targets for in vitro pharmacological profiling. In
the past decades, the rapid progress in emerging functional assays and instrumentation has enabled to
readily monitor thermoTRP channel activity, and to develop high throughput screening (HTS) assays for
TPR drug discovery. Chronologically, functional methods for ion channels include the ligand binding assay,
flux-based assay, electrophysiology, fluorescence-based assays, and, more recently, automated electrophysiological assays. Here we described the methodology used to monitor the functionality of two thermoTRPs, TRPV1 and TRPM8, based on Ca2+ microfluorography using a 96-well fluorescence plate reader that allows the implementation of a medium- to high-throughput format ideal for drug screening.
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Keywords/Subjects: Ion channels High-throughput screening Ligand binding assay Flux-based assay Fluorescence- based assay Drug discovery |
Type of document: application/pdf |
Access rights: info:eu-repo/semantics/closedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI: https://doi.org/10.1007/978-1-4939-9446-5 |
Appears in Collections: Artículos Fisiología
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