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Chapter 6: Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique


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Title:
Chapter 6: Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique
Authors:
De la Peña García, Elvira  
Gomis García, Ana María
Editor:
Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature.
Department:
Departamentos de la UMH::Fisiología
Instituto de Neurociencias
Issue Date:
2019
URI:
https://hdl.handle.net/11000/30739
Abstract:
Transient receptor potential (TRP) ion channels are involved in a variety of fundamental physiological processes, and their malfunction produces numerous human diseases. Therefore, these proteins represent a class of attractive drug targets and a class of important off-targets for in vitro pharmacological profiling. In the past decades, the rapid progress in emerging functional assays and instrumentation has enabled to readily monitor thermoTRP channel activity, and to develop high throughput screening (HTS) assays for TPR drug discovery. Chronologically, functional methods for ion channels include the ligand binding assay, flux-based assay, electrophysiology, fluorescence-based assays, and, more recently, automated electrophysiological assays. Here we described the methodology used to monitor the functionality of two thermoTRPs, TRPV1 and TRPM8, based on Ca2+ microfluorography using a 96-well fluorescence plate reader that allows the implementation of a medium- to high-throughput format ideal for drug screening.
Keywords/Subjects:
Ion channels
High-throughput screening
Ligand binding assay
Flux-based assay
Fluorescence- based assay
Drug discovery
Type of document:
info:eu-repo/semantics/bookPart
Access rights:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI:
https://doi.org/10.1007/978-1-4939-9446-5
Published in:
TRP Channels Methods and Protocols Edited by Antonio Ferrer-Montiel Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, Alicante, Spain Tim Hucho Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9445-8 ISBN 978-1-4939-9446-5 (eBook) © Springer Science+Business Media, LLC, part of Springer Nature 2019
Appears in Collections:
Artículos Fisiología



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