Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/30734
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dc.contributor.authorVázquez Guilló, Rebeca-
dc.contributor.authorMartínez Tomé, María José-
dc.contributor.authorKahveci, Zehra-
dc.contributor.authorTorres, Iván-
dc.contributor.authorFalcó, Alberto-
dc.contributor.authorMallavia, Ricardo-
dc.contributor.authorMateo , C. Reyes-
dc.contributor.otherDepartamentos de la UMH::Agroquímica y Medio Ambientees_ES
dc.date.accessioned2024-01-26T10:42:12Z-
dc.date.available2024-01-26T10:42:12Z-
dc.date.created2018-08-20-
dc.identifier.citationPolymers 2018, 10, 938es_ES
dc.identifier.issn2073-4360-
dc.identifier.urihttps://hdl.handle.net/11000/30734-
dc.description.abstractIn the present work, we have synthesized a novel green-emitter conjugated polyelectrolyte Copoly-{[9,9-bis(60-N,N,N-trimethylammonium)hexyl]-2,7-(fluorene)-alt-4,7-(2-(phenyl) benzo[d] [1,2,3] triazole)} bromide (HTMA-PFBT) by microwave-assisted Suzuki coupling reaction. Its fluorescent properties have been studied in aqueous media and in presence of model membranes of different composition, in order to explore its ability to be used as a green fluorescent membrane probe. The polyelectrolyte was bound with high affinity to the membrane surface, where it exhibited high fluorescence efficiency and stability. HTMA-PFBT showed lower affinity to zwitterionic membranes as compared to anionic ones, as well as a more external location, near the membrane-aqueous interface. Fluorescence microscopy studies confirmed the interaction of HTMA-PFBT with the model membranes, labelling the lipid bilayer without perturbing its morphology and showing a clear preference towards anionic systems. In addition, the polyelectrolyte was able to label the membrane of bacteria and living mammalian cells, separately. Finally, we explored if the polyelectrolyte can function also as a sensitive probe able of detecting lipid-phase transitions. All these results suggest the potential use of HTMA-PFBT as a green membrane marker for bioimaging and selective recognition of bacteria cell over mammalian ones and as a tool to monitor changes in physical state of lipid membranes.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent20es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectpolyfluorenees_ES
dc.subjectconjugated polyelectrolytees_ES
dc.subjectbioimaginges_ES
dc.subjectfluorescent membrane probees_ES
dc.subjectbioimaginges_ES
dc.subject.classificationQuímica Físicaes_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::54 - Químicaes_ES
dc.titleSynthesis and Characterization of a Novel Green Cationic Polyfluorene and Its Potential Use as a Fluorescent Membrane Probees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.3390/polym10090938es_ES
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Artículos Agroquímica y Medio Ambiente


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