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The Effect of Germanium‐Loaded Hydroxyapatite Biomaterials on Bone Marrow Mesenchymal Stem Cells Growth


 2022 Cells-JElango.pdf

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The Effect of Germanium‐Loaded Hydroxyapatite Biomaterials on Bone Marrow Mesenchymal Stem Cells Growth
Elango, Jeevithan
Bushin, Rodion
Lijnev, Artiom
De Aza, Piedad  
Pérez-Albacete Martínez, Carlos  
Granero Marin, Jose Manuel  
Hernandez, Ana Belen
Meseguer-Olmo, Luis  
Maté Sánchez de Va´, José Eduardo  
Issue Date:
Hydroxyapatite (HA) is a hard mineral component of mineralized tissues, mainly composed of calcium and phosphate. Due to its bioavailability, HA is potentially used for the repair and regeneration of mineralized tissues. For this purpose, the properties of HA are significantly improved by adding natural and synthetic materials. In this sense, the germanium (Ge) mineral was loaded in HA biomaterial by cold isostatic pressure for the first time and characterization and biocompatibility using bone marrow mesenchymal stem cells (BM-MSCs) were investigated. The addition of Ge at 5% improved the solubility (3.32%), stiffness (18.34 MPa), water holding (31.27%) and biodegradation (21.87%) properties of HA, compared to control. Compared to all composite biomaterials, the drug-releasing behavior of HA-3% Ge was higher at pH 1 and 3 and the maximum drug release was obtained at pH 7 and 9 with HA-5% Ge biomaterials. Among the different mediums tested, the DMEM-medium showed a higher drug release rate, especially at 60 min. HA-Ge biomaterials showed better protein adhesion and apatite layer formation, which ultimately proves the compatibility in BM-MSCs culture. Except for higher concentrations of HA (5 and 10 mg/mL), the different concentrations of Ge and HA and wells coated with 1% of HA-1% Ge had higher BM-MSCs growth than control. All these findings concluded that the fabricated HA biomaterials loaded with Ge could be the potential biomaterial for culturing mammalian cells towards mineralized tissue repair and regeneration.
drug release
mesenchymal stem cells
protein adhesion
Knowledge area:
CDU: Ciencias aplicadas: Ingeniería. Tecnología
Type of document:
Access rights:
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Appears in Collections:
Instituto de Bioingeniería

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