Preparation, characterization and in vitro behavior of a new eutectoidbioceramic.

Abstract

2014AbstractA new type of bioceramic has been designed and obtained within the sub-system Ca2SiO4–7CaOP2O52SiO2. The selected composition wasthat corresponding to the eutectoid point 69 wt% dicalcium silicate–31 wt% tricalcium phosphate. Sintering behavior, phase evolution andmicrostructural changes were analyzed by XRD and SEM. Bio-reactivity was determined by immersion of materials in simulated body fluid forseveral periods of time as well as studies in human adipose stem cells (hASC). The investigated materials are bio-acceptable since no toxic or otherharmful evidence was detected. A carbonated hydroxyapatite was formed on the surface of the 31R material within 3 days. Cell attachment assayshowed that the ceramics supported the hASC cells adhesion and spreading, and the cells established close contacts with the ceramics after 24 h ofculture. The influence of the microstructure (porosity, grain size and phase composition) on the in vitro behavior of the obtained bioceramics wasalso examined.