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dc.contributor.authorPuente Marín, Sara-
dc.contributor.authorNombela Díaz, Iván-
dc.contributor.authorChico, Verónica-
dc.contributor.authorCiordia, Sergio-
dc.contributor.authorMena, María Carmen-
dc.contributor.authorColl, Julio-
dc.contributor.authorMercado, Luis-
dc.contributor.authorOrtega-Villaizan, María del Mar-
dc.date.accessioned2021-01-25T10:36:54Z-
dc.date.available2021-01-25T10:36:54Z-
dc.date.created2018-10-08-
dc.date.issued2021-01-25-
dc.identifier.issn1664-3224-
dc.identifier.urihttp://hdl.handle.net/11000/7004-
dc.description.abstractFish red blood cells (RBCs), are integral in several biologic processes relevant to immunity, such as pathogen recognition, pathogen binding and clearance, and production of effector molecules and cytokines. So far, one of the best strategies to control and prevent viral diseases in aquaculture is DNA immunization. DNA vaccines (based on the rhabdoviral glycoprotein G [gpG] gene) have been shown to be effective against fish rhabdoviruses. However, more knowledge about the immune response triggered by DNA immunization is necessary to develop novel and more effective strategies. In this study, we investigated the role of fish RBCs in immune responses induced by DNA vaccines. We show for the first time that rainbow trout RBCs express gpG of viral hemorrhagic septicaemia virus (VHSV) (GVHSV) when transfected with the DNA vaccine ex vivo and modulate the expression of immune genes and proteins. Functional network analysis of transcriptome profiling of RBCs expressing GVHSV revealed changes in gene expression related to G-protein coupled receptor (GPCR)-downstream signaling, complement activation, and RAR related orphan receptor a (RORA). Proteomic profile functional network analysis of GVHSV-transfected RBCs revealed proteins involved in the detoxification of reactive oxygen species, interferon-stimulated gene 15 (ISG15) antiviral mechanisms, antigen presentation of exogenous peptides, and the proteasome. Conditioned medium of GVHSV-transfected RBCs conferred antiviral protection and induced ifn1 and mx gene expression in RTG-2 cells infected with VHSV. In summary, rainbow trout nucleated RBCs could be actively participating in the regulation of the fish immune response to GVHSV DNA vaccine, and thus may represent a possible carrier cells for the development of new vaccine approaches.es
dc.description.sponsorshipThis work was supported by the European Research Council (ERC Starting Grant GA639249)-
dc.description.sponsorshipThe proteomic analysis was performed in the Proteomics Facility of The Spanish National Center for Biotechnology (CNB-CSIC) belonging to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019-
dc.description.sponsorshipThis work was supported by the European Research Council (ERC Starting Grant GA639249).-
dc.description.sponsorshipThe proteomic analysis was performed in the Proteomics Facility of The Spanish National Center for Biotechnology (CNB-CSIC) of ProteoRed, PRB3- ISCIII, supported by grant PT17/0019-
dc.formatapplication/pdfes
dc.format.extent14es
dc.language.isoenges
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectrainbow trout,es
dc.subjecterythrocyteses
dc.subjectred blood cellses
dc.subjectVHSV glycoprotein Ges
dc.subjectDNA vaccinees
dc.subjectTranscriptomees
dc.subjectproteomees
dc.subjectimmune responsees
dc.subject.other577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleRainbow Trout Erythrocytes ex vivo Transfection With a DNA Vaccine Encoding VHSV Glycoprotein G Induces an Antiviral Immune Responsees
dc.typeinfo:eu-repo/semantics/articlees
dc.contributor.instituteInstituto de Biología Molecular y Celulares
dc.identifier.doi10.3390/cells8050386-
dc.identifier.doi10.3389/fimmu.2018.02477-
dc.relation.publisherversionhttps://doi.org/10.3390/cells8050386-
dc.relation.publisherversionhttps://doi.org/10.3389/fimmu.2018.02477-
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