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dc.contributor.authorBech, Federico-
dc.contributor.authorGonzález González, Omar-
dc.contributor.authorArtime, Enol-
dc.contributor.authorSerrano, Joana-
dc.contributor.authorAlcalde, Ignacio-
dc.contributor.authorGallar Martínez, Juana-
dc.contributor.authorMerayo-Lloves, Jesús-
dc.contributor.authorBelmonte Martínez, Carlos-
dc.contributor.otherDepartamentos de la UMH::Fisiologíaes
dc.date.accessioned2019-07-09T06:56:30Z-
dc.date.available2019-07-09T06:56:30Z-
dc.date.created2018-04-04-
dc.date.issued2019-07-09-
dc.identifier.issn0146-0404-
dc.identifier.issn1552-5783-
dc.identifier.urihttp://hdl.handle.net/11000/5231-
dc.description.abstractPURPOSE. To define the characteristics and time course of the morphologic and functional changes experienced by corneal sensory nerves after photorefractive keratectomy (PRK). METHODS. Unilateral corneal excimer laser photoablation was performed in 54 anesthetized 3- to 6-month-old mice; 11 na¨ıve animals served as control. Mice were killed 0, 3, 7, 15, and 30 days after PRK. Excised eyes were placed in a recording chamber superfused at 348C. Electrical nerve impulse activity of single sensory terminals was recorded with a micropipette applied onto the corneal surface. Spontaneous and stimulus-evoked (cold, heat, mechanical, and chemical stimuli) nerve terminal impulse (NTI) activity was analyzed. Corneas were fixed and stained with anti–b-Tubulin III antibody to measure nerve density and number of epithelial nerve penetration points of regenerating subbasal leashes. RESULTS. Nerve fibers and NTI activity were absent in the injured area between 0 and 7 days after PRK, when sparse regenerating nerve sprouts appear. On day 15, subbasal nerve density reached half the control value and abnormally responding cold-sensitive terminals were recorded inside the lesion. Thirty days after PRK, nerve density was almost restored, active cold thermoreceptors were abundant, and polymodal nociceptor activity first reappeared. CONCLUSIONS. Morphologic regeneration of subbasal corneal nerves started shortly after PRK ablation and was substantially completed 30 days later. Functional recovery appears faster in cold terminals than polymodal terminals, possibly reflecting an incomplete damage of the more extensively branched cold-sensitive axon terminals. Evolution of postsurgical discomfort sensations quality may be associated with the variable regeneration pattern of each fiber typees
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dc.format.extent12es
dc.language.isoenges
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectnerve regenerationes
dc.subjectphotorefractive keratectomyes
dc.subjectcold thermoreceptorses
dc.subjectpolymodales
dc.subjectdysesthesiaes
dc.subject.otherFisiologíaes
dc.titleFunctional and Morphologic Alterations in Mechanical, Polymodal, and Cold Sensory Nerve Fibers of the Cornea Following Photorefractive Keratectomyes
dc.typeinfo:eu-repo/semantics/articlees
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