Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/31209
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dc.contributor.authorGodoy, Pamela A.-
dc.contributor.authorMennickent, Daniela-
dc.contributor.authorCuchillo‑Ibañez, Inmaculada-
dc.contributor.authorRamirez Molina, Oscar-
dc.contributor.authorSilva-Grecchi, Tiare-
dc.contributor.authorPanes Fernandez, Jessica-
dc.contributor.authorCastro, Patricio-
dc.contributor.authorSáez-Valero, Javier-
dc.contributor.authorFuentealba, Jorge-
dc.date.accessioned2024-02-07T10:22:40Z-
dc.date.available2024-02-07T10:22:40Z-
dc.date.created2021-07-31-
dc.identifier.citationBiomed Pharmacother. 2021 Oct:142:111968es_ES
dc.identifier.issn1950-6007-
dc.identifier.issn0753-3322-
dc.identifier.urihttps://hdl.handle.net/11000/31209-
dc.description.abstractAmyloid beta peptide (Aβ) is tightly associated with the physiopathology of Alzheimer’s Disease (AD) as one of the most important factors in the evolution of the pathology. In this context, we previously reported that Aβ increases the expression of ionotropic purinergic receptor 2 (P2×2R). However, its role on the cellular and molecular Aβ toxicity is unknown, especially in human brain of AD patients. Using cellular and molecular approaches in hippocampal neurons, PC12 cells, and human brain samples of patients with AD, we evaluated the participation of P2×2R in the physiopathology of AD. Here, we reported that Aβ oligomers (Aβo) increased P2×2 levels in mice hippocampal neurons, and that this receptor increases at late Braak stages of AD patients. Aβo also increases the colocalization of APP with Rab5, an early endosomes marker, and decreased the nuclear/cytoplasmic ratio of Fe65 and PGC-1α immunoreactivity. The overexpression in PC12 cells of P2×2a, but not P2×2b, replicated these changes in Fe65 and PGC-1α; however, both overexpressed isoforms increased levels of Aβ. Taken together, these data suggest that P2×2 is upregulated in AD and it could be a key potentiator of the physiopathology of Aβ. Our results point to a possible participation in a toxic cycle that increases Aβ production, Ca2+ overload, and a decrease of PGC-1α. These novel findings put the P2×2R as a key novel pharmacological target to develop new therapeutic strategies to treat Alzheimer’s Disease.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent15es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectP2×2es_ES
dc.subjectPurinergic receptorses_ES
dc.subjectAmyloid betaes_ES
dc.subjectAlzheimer’s diseasees_ES
dc.subjectFe65es_ES
dc.subjectAPPes_ES
dc.titleIncreased P2×2 receptors induced by amyloid-β peptide participates in the neurotoxicity in alzheimer’s diseasees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.contributor.instituteInstitutos de la UMH::Instituto de Neurocienciases_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.biopha.2021.111968es_ES
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Instituto de Neurociencias


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