Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/11000/31209

Increased P2×2 receptors induced by amyloid-β peptide participates in the neurotoxicity in alzheimer’s disease

Título :
Increased P2×2 receptors induced by amyloid-β peptide participates in the neurotoxicity in alzheimer’s disease
Autor :
Godoy, Pamela A.
Mennickent, Daniela  
Cuchillo‑Ibañez, Inmaculada
Ramirez Molina, Oscar  
Silva-Grecchi, Tiare
Panes Fernandez, Jessica  
Castro, Patricio  
Sáez-Valero, Javier  
Fuentealba, Jorge  
Editor :
Elsevier
Fecha de publicación:
2021-07-31
URI :
https://hdl.handle.net/11000/31209
Resumen :
Amyloid beta peptide (Aβ) is tightly associated with the physiopathology of Alzheimer’s Disease (AD) as one of the most important factors in the evolution of the pathology. In this context, we previously reported that Aβ increases the expression of ionotropic purinergic receptor 2 (P2×2R). However, its role on the cellular and molecular Aβ toxicity is unknown, especially in human brain of AD patients. Using cellular and molecular approaches in hippocampal neurons, PC12 cells, and human brain samples of patients with AD, we evaluated the participation of P2×2R in the physiopathology of AD. Here, we reported that Aβ oligomers (Aβo) increased P2×2 levels in mice hippocampal neurons, and that this receptor increases at late Braak stages of AD patients. Aβo also increases the colocalization of APP with Rab5, an early endosomes marker, and decreased the nuclear/cytoplasmic ratio of Fe65 and PGC-1α immunoreactivity. The overexpression in PC12 cells of P2×2a, but not P2×2b, replicated these changes in Fe65 and PGC-1α; however, both overexpressed isoforms increased levels of Aβ. Taken together, these data suggest that P2×2 is upregulated in AD and it could be a key potentiator of the physiopathology of Aβ. Our results point to a possible participation in a toxic cycle that increases Aβ production, Ca2+ overload, and a decrease of PGC-1α. These novel findings put the P2×2R as a key novel pharmacological target to develop new therapeutic strategies to treat Alzheimer’s Disease.
Palabras clave/Materias:
P2×2
Purinergic receptors
Amyloid beta
Alzheimer’s disease
Fe65
APP
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI :
https://doi.org/10.1016/j.biopha.2021.111968
Aparece en las colecciones:
Instituto de Neurociencias



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.