Please use this identifier to cite or link to this item: https://hdl.handle.net/11000/31149
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCHICO GRAS, VERONICA-
dc.contributor.authorGomez, Noemi-
dc.contributor.authorESTEPA, AMPARO-
dc.contributor.authorPerez, Luis-
dc.date.accessioned2024-02-06T12:13:49Z-
dc.date.available2024-02-06T12:13:49Z-
dc.date.created2005-10-
dc.identifier.citationJournal of Virological Methods Volume 132, Issues 1–2, March 2006, Pages 154-159es_ES
dc.identifier.issn0166-0934-
dc.identifier.urihttps://hdl.handle.net/11000/31149-
dc.description.abstractA quantitative real-time RT-PCR (Q-RT-PCR) was developed to detect and determine the amount of viral hemorrhagic septicemia virus (VHSV) in organs of experimentally infected rainbow trout. Primers and TaqMan probes targeting the glycoprotein (G) and the nucleoprotein (N) genes of the virus were designed. The efficiency, linear range and detection limit of the Q-RT-PCR were assessed on cell cultured virus samples. VHSV N gene amplification was more efficient and more sensitive than the VHSV G amplicon. On cell culture grown virus, samples could be accurately assayed over a range of seven logs of infectious particles per reaction. To demonstrate the utility of Q-RT-PCR in vivo, bath infection trials were carried out and samples from fish spleen, kidney, liver and blood were harvested and tested for VHSV. Q-RT-PCR was a more reliable method than either conventional RT-PCR or the cell culture assay for virus diagnosis. Results of VHSV RNA detection in fish shortly after infection as well as on asymptomatic fish several weeks after experimental challenge are presented here. This is the first report showing the utility of Q-RT-PCR for VHSV detection and quantitation both in vitro and in vivo. The suitability of this method to test the efficacy of antiviral treatments is also discussed.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent6es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectVHSVes_ES
dc.subjectReal-time PCRes_ES
dc.subjectFish diseaseses_ES
dc.subjectVirus detectiones_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::573 - Biología general y teóricaes_ES
dc.titleRapid detection and quantitation of viral hemorrhagic septicemia virus in experimentally challenged rainbow trout by real-time RT-PCRes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.contributor.instituteInstitutos de la UMH::Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elchees_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.jviromet.2005.10.005es_ES
Appears in Collections:
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche


no-thumbnailView/Open:

 JVirologyMethods 2006-main (1).pdf



154,94 kB
Adobe PDF
Share:


Creative Commons ???jsp.display-item.text9???