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Expression and antiviral activity of a β-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences
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Título : Expression and antiviral activity of a β-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences |
Autor : Falco, Antonio CHICO GRAS, VERONICA Marroquí, L. Perez, L. Coll, J.M. Estepa, A. |
Editor : Elsevier |
Fecha de publicación: 2007-06 |
URI : https://hdl.handle.net/11000/31148 |
Resumen :
The in silico identification of a β-defensin-like peptide sequence (omBD-1) in the rainbow trout (Oncorhynchuss mykiss) database of salmonid EST is reported here. We have studied the transcript expression of this β-defensin-like sequence in different organs and expressed the recombinant peptide in a fish cell line. Finally, we have demonstrated the in vitro antiviral activity of the recombinant trout β-defensin-like peptide against viral haemorrhagic septicaemia rhabdovirus (VHSV), one of the most devastating viruses for worldwide aquaculture. Thus, the resistance to VHSV infection of EPC cells transfected with pMCV 1.4-omBD-1 has been shown. Since EPC cells transfected with omBD-1 produced acid and heat stable antiviral activity and up regulation of Mx, a type I IFN-mediated mechanism of antiviral action is suggested. To our knowledge, this is the first report showing biological activity of a β-defensin-like peptide from any fish.
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Palabras clave/Materias: Antimicrobial Defensin VHSV IFN Mx |
Área de conocimiento : CDU: Ciencias puras y naturales: Biología: Biología general y teórica |
Tipo documento : application/pdf |
Derechos de acceso: info:eu-repo/semantics/closedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
DOI : https://doi.org/10.1016/j.molimm.2007.06.358 |
Aparece en las colecciones: Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche
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La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.