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Expression and antiviral activity of a β-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences


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Título :
Expression and antiviral activity of a β-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences
Autor :
Falco, Antonio  
CHICO GRAS, VERONICA  
Marroquí, L.
Perez, L.
Coll, J.M.
Estepa, A.
Editor :
Elsevier
Fecha de publicación:
2007-06
URI :
https://hdl.handle.net/11000/31148
Resumen :
The in silico identification of a β-defensin-like peptide sequence (omBD-1) in the rainbow trout (Oncorhynchuss mykiss) database of salmonid EST is reported here. We have studied the transcript expression of this β-defensin-like sequence in different organs and expressed the recombinant peptide in a fish cell line. Finally, we have demonstrated the in vitro antiviral activity of the recombinant trout β-defensin-like peptide against viral haemorrhagic septicaemia rhabdovirus (VHSV), one of the most devastating viruses for worldwide aquaculture. Thus, the resistance to VHSV infection of EPC cells transfected with pMCV 1.4-omBD-1 has been shown. Since EPC cells transfected with omBD-1 produced acid and heat stable antiviral activity and up regulation of Mx, a type I IFN-mediated mechanism of antiviral action is suggested. To our knowledge, this is the first report showing biological activity of a β-defensin-like peptide from any fish.
Palabras clave/Materias:
Antimicrobial
Defensin
VHSV
IFN
Mx
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología: Biología general y teórica
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI :
https://doi.org/10.1016/j.molimm.2007.06.358
Aparece en las colecciones:
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.