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dc.contributor.authorArias, Sagrario-
dc.contributor.authorSandoval, Ángel-
dc.contributor.authorArcos, Mario-
dc.contributor.authorCañedo, Librada María-
dc.contributor.authorMaestro García-Donas, Beatriz-
dc.contributor.authorSanz, Jesús M.-
dc.contributor.authorNaharro, Germán-
dc.contributor.authorLuengo, José M.-
dc.date.accessioned2024-02-02T09:44:29Z-
dc.date.available2024-02-02T09:44:29Z-
dc.date.created2008-
dc.identifier.citationMicrobial Biotechnology . 2008 Mar;1(2):170-6.es_ES
dc.identifier.issn1751-7915-
dc.identifier.urihttps://hdl.handle.net/11000/30959-
dc.description.abstractThe substrate specificity of the two polymerases (PhaC1 and PhaC2) involved in the biosynthesis of medium-chain-length poly-hydroxyalkanoates (mcl PHAs) in Pseudomonas putida U has been studied in vivo. For these kind of experiments, two recombinant strains derived from a genetically engineered mutant in which the whole pha locus had been deleted (P. putida U Δpha) were employed. These bacteria, which expresses only phaC1 (P. putida U Δpha pMC-phaC1) or only phaC2 (P. putida U Δpha pMC-phaC2), accumulated different PHAs in function of the precursor supplemented to the culture broth. Thus, the P. putida U Δpha pMC-phaC1 strain was able to synthesize several aliphatic and aromatic PHAs when hexanoic, heptanoic, octanoic decanoic, 5-phenylvaleric, 6-phenylhexanoic, 7-phenylheptanoic, 8-phenyloctanoic or 9-phenylnonanoic acid were used as precursors; the highest accumulation of polymers was observed when the precursor used were decanoic acid (aliphatic PHAs) or 6-phenylhexanoic acid (aromatic PHAs). However, although it synthesizes similar aliphatic PHAs (the highest accumulation was observed when hexanoic acid was the precursor) the other recombinant strain (P. putida U Δpha pMC-phaC2) only accumulated aromatic PHAs when the monomer to be polymerized was 3-hydroxy-5-phenylvaleryl-CoA. The possible influence of the putative three-dimensional structures on the different catalytic behaviour of PhaC1 and PhaC2 is discussed.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent8es_ES
dc.language.isoenges_ES
dc.publisherWilleyes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlePoly-3-hydroxyalkanoate synthases from Pseudomonas putida U: substrate specificity and ultrastructural studieses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.contributor.instituteInstitutos de la UMH::Instituto de Bioingenieríaes_ES
dc.relation.publisherversionhttps://doi.org/10.1111/j.1751-7915.2007.00016.xes_ES
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