Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/11000/30901

Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems


no-thumbnailVer/Abrir:

 Affinity partitioning of proteins tagged with choline-binding modules in.pdf



1,5 MB
Adobe PDF
Compartir:

Este recurso está restringido

Título :
Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
Autor :
Maestro García-Donas, Beatriz
Velasco, Isabel
Castillejo, Isabel
Arévalo-Rodríguez, Miguel
Cebolla, Ángel
Sanz, Jesús M
Editor :
Elsevier
Fecha de publicación:
2008
URI :
https://hdl.handle.net/11000/30901
Resumen :
We present a novel procedure for affinity partitioning of recombinant proteins fused to the cholinebinding module C-LytA in aqueous two-phase systems containing poly(ethylene glycol) (PEG). Proteins tagged with the C-LytA module and exposed to the two-phase systems are quantitatively localized in the PEG-rich phase, whereas subsequent addition of the natural ligand choline specifically shifts their localization to the PEG-poor phase by displacement of the polymer from the binding sites. The described procedure is simple, scalable and reproducible, and has been successfully applied to the purification of four diverse proteins, resulting in high yields and purity.
Palabras clave/Materias:
Aqueous two-phase systems
Protein purification
Poly(ethylene glycol)
Choline-binding module
Liquid–liquid extraction
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/closedAccess
DOI :
https://doi.org/10.1016/j.chroma.2008.08.106
Aparece en las colecciones:
Instituto de Bioingeniería



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.